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Comparison of MIC Test Strip and Sensititre YeastOne with the CLSI and EUCAST Broth Microdilution Reference Methods for In Vitro Antifungal Susceptibility Testing of Cryptococcus neoformans

We compared MIC test strip (MTS) and Sensititre YeastOne (SYO) methods with EUCAST and CLSI methods for amphotericin B, 5-fluocytosine, fluconazole, voriconazole, and isavuconazole against 106 isolates. The overall essential agreement between the EUCAST and CLSI methods was >72% and >94% at ±1...

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Published in:Antimicrobial agents and chemotherapy 2020-03, Vol.64 (4)
Main Authors: Delma, Fatima Zohra, Al-Hatmi, Abdullah M S, Buil, Jochem B, van der Lee, Hein, Tehupeiory-Kooreman, Marlou, de Hoog, G Sybren, Meletiadis, Joseph, Verweij, Paul E
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cited_by cdi_FETCH-LOGICAL-a418t-29294e7fc49df09c5747da305c7c815a779116347914c0754c567054aaa2b9fd3
cites cdi_FETCH-LOGICAL-a418t-29294e7fc49df09c5747da305c7c815a779116347914c0754c567054aaa2b9fd3
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container_title Antimicrobial agents and chemotherapy
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creator Delma, Fatima Zohra
Al-Hatmi, Abdullah M S
Buil, Jochem B
van der Lee, Hein
Tehupeiory-Kooreman, Marlou
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Meletiadis, Joseph
Verweij, Paul E
description We compared MIC test strip (MTS) and Sensititre YeastOne (SYO) methods with EUCAST and CLSI methods for amphotericin B, 5-fluocytosine, fluconazole, voriconazole, and isavuconazole against 106 isolates. The overall essential agreement between the EUCAST and CLSI methods was >72% and >94% at ±1 and ±2 dilutions, respectively. The essential agreements between SYO and EUCAST/CLSI for amphotericin B, 5-flucytosine, fluconazole, and voriconazole were >89/>93% and between MTS and EUCAST/CLSI were >57/>75%. Very major error rates were low for amphotericin B and fluconazole (
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subjects Antifungal Agents - pharmacology
Cryptococcosis - microbiology
Cryptococcus neoformans - drug effects
Cryptococcus neoformans - isolation & purification
Humans
Microbial Sensitivity Tests - methods
Microbial Sensitivity Tests - standards
Susceptibility
Time Factors
title Comparison of MIC Test Strip and Sensititre YeastOne with the CLSI and EUCAST Broth Microdilution Reference Methods for In Vitro Antifungal Susceptibility Testing of Cryptococcus neoformans
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