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Comparison of MIC Test Strip and Sensititre YeastOne with the CLSI and EUCAST Broth Microdilution Reference Methods for In Vitro Antifungal Susceptibility Testing of Cryptococcus neoformans
We compared MIC test strip (MTS) and Sensititre YeastOne (SYO) methods with EUCAST and CLSI methods for amphotericin B, 5-fluocytosine, fluconazole, voriconazole, and isavuconazole against 106 isolates. The overall essential agreement between the EUCAST and CLSI methods was >72% and >94% at ±1...
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Published in: | Antimicrobial agents and chemotherapy 2020-03, Vol.64 (4) |
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container_title | Antimicrobial agents and chemotherapy |
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creator | Delma, Fatima Zohra Al-Hatmi, Abdullah M S Buil, Jochem B van der Lee, Hein Tehupeiory-Kooreman, Marlou de Hoog, G Sybren Meletiadis, Joseph Verweij, Paul E |
description | We compared MIC test strip (MTS) and Sensititre YeastOne (SYO) methods with EUCAST and CLSI methods for amphotericin B, 5-fluocytosine, fluconazole, voriconazole, and isavuconazole against 106
isolates. The overall essential agreement between the EUCAST and CLSI methods was >72% and >94% at ±1 and ±2 dilutions, respectively. The essential agreements between SYO and EUCAST/CLSI for amphotericin B, 5-flucytosine, fluconazole, and voriconazole were >89/>93% and between MTS and EUCAST/CLSI were >57/>75%. Very major error rates were low for amphotericin B and fluconazole ( |
doi_str_mv | 10.1128/AAC.02261-19 |
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isolates. The overall essential agreement between the EUCAST and CLSI methods was >72% and >94% at ±1 and ±2 dilutions, respectively. The essential agreements between SYO and EUCAST/CLSI for amphotericin B, 5-flucytosine, fluconazole, and voriconazole were >89/>93% and between MTS and EUCAST/CLSI were >57/>75%. 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subjects | Antifungal Agents - pharmacology Cryptococcosis - microbiology Cryptococcus neoformans - drug effects Cryptococcus neoformans - isolation & purification Humans Microbial Sensitivity Tests - methods Microbial Sensitivity Tests - standards Susceptibility Time Factors |
title | Comparison of MIC Test Strip and Sensititre YeastOne with the CLSI and EUCAST Broth Microdilution Reference Methods for In Vitro Antifungal Susceptibility Testing of Cryptococcus neoformans |
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