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Evaluation of ID Fungi Plates Medium for Identification of Molds by MALDI Biotyper
MALDI-TOF mass spectrometry (MS) identification of pathogenic filamentous fungi is often impaired by difficulties in harvesting hyphae embedded in the medium and long extraction protocols. The ID Fungi Plate (IDFP) is a novel culture method developed to address such difficulties and improve the iden...
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Published in: | Journal of clinical microbiology 2020-04, Vol.58 (5) |
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container_title | Journal of clinical microbiology |
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creator | Robert, Marie Gladys Romero, Charlotte Dard, Céline Garnaud, Cécile Cognet, Odile Girard, Thomas Rasamoelina, Tahinamandranto Cornet, Muriel Maubon, Danièle |
description | MALDI-TOF mass spectrometry (MS) identification of pathogenic filamentous fungi is often impaired by difficulties in harvesting hyphae embedded in the medium and long extraction protocols. The ID Fungi Plate (IDFP) is a novel culture method developed to address such difficulties and improve the identification of filamentous fungi by MALDI-TOF MS. We cultured 64 strains and 11 clinical samples on IDFP, Sabouraud agar-chloramphenicol (SAB), and ChromID Candida agar (CAN2). We then compared the three media for growth, ease of harvest, amount of material picked, and MALDI-TOF identification scores after either rapid direct transfer (DT) or a long ethanol-acetonitrile (EA) extraction protocol. Antifungal susceptibility testing and microscopic morphology after subculture on SAB and IDFP were also compared for ten molds. Growth rates and morphological aspects were similar for the three media. With IDFP, harvesting of fungal material for the extraction procedure was rapid and easy in 92.4% of cases, whereas it was tedious on SAB or CAN2 in 65.2% and 80.3% of cases, respectively. The proportion of scores above 1.7 (defined as acceptable identification) were comparable for both extraction protocols using IDFP (
= 0.256). Moreover, rates of acceptable identification after DT performed on IDFP (93.9%) were significantly higher than those obtained after EA extraction with SAB (69.7%) or CAN2 (71.2%) (
= |
doi_str_mv | 10.1128/jcm.01687-19 |
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= 0.256). Moreover, rates of acceptable identification after DT performed on IDFP (93.9%) were significantly higher than those obtained after EA extraction with SAB (69.7%) or CAN2 (71.2%) (
= <0.001 and
= 0.001, respectively). Morphological aspects and antifungal susceptibility testing were similar between IDFP and SAB. IDFP is a culture plate that facilitates and improves the identification of filamentous fungi, allowing accurate routine identification of molds with MALDI-TOF-MS using a rapid-extraction protocol.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/jcm.01687-19</identifier><identifier>PMID: 32051262</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Life Sciences ; Microbiology and Parasitology ; Mycology</subject><ispartof>Journal of clinical microbiology, 2020-04, Vol.58 (5)</ispartof><rights>Copyright © 2020 American Society for Microbiology.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Copyright © 2020 American Society for Microbiology. 2020 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-f3a71c439582cecec30ca85caa28e522cd630191953bcf77cd904459552035253</citedby><cites>FETCH-LOGICAL-c484t-f3a71c439582cecec30ca85caa28e522cd630191953bcf77cd904459552035253</cites><orcidid>0000-0003-4155-3675</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180245/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180245/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32051262$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03703158$$DView record in HAL$$Hfree_for_read</backlink></links><search><contributor>Hanson, Kimberly E.</contributor><creatorcontrib>Robert, Marie Gladys</creatorcontrib><creatorcontrib>Romero, Charlotte</creatorcontrib><creatorcontrib>Dard, Céline</creatorcontrib><creatorcontrib>Garnaud, Cécile</creatorcontrib><creatorcontrib>Cognet, Odile</creatorcontrib><creatorcontrib>Girard, Thomas</creatorcontrib><creatorcontrib>Rasamoelina, Tahinamandranto</creatorcontrib><creatorcontrib>Cornet, Muriel</creatorcontrib><creatorcontrib>Maubon, Danièle</creatorcontrib><title>Evaluation of ID Fungi Plates Medium for Identification of Molds by MALDI Biotyper</title><title>Journal of clinical microbiology</title><addtitle>J Clin Microbiol</addtitle><description>MALDI-TOF mass spectrometry (MS) identification of pathogenic filamentous fungi is often impaired by difficulties in harvesting hyphae embedded in the medium and long extraction protocols. The ID Fungi Plate (IDFP) is a novel culture method developed to address such difficulties and improve the identification of filamentous fungi by MALDI-TOF MS. We cultured 64 strains and 11 clinical samples on IDFP, Sabouraud agar-chloramphenicol (SAB), and ChromID Candida agar (CAN2). We then compared the three media for growth, ease of harvest, amount of material picked, and MALDI-TOF identification scores after either rapid direct transfer (DT) or a long ethanol-acetonitrile (EA) extraction protocol. Antifungal susceptibility testing and microscopic morphology after subculture on SAB and IDFP were also compared for ten molds. Growth rates and morphological aspects were similar for the three media. With IDFP, harvesting of fungal material for the extraction procedure was rapid and easy in 92.4% of cases, whereas it was tedious on SAB or CAN2 in 65.2% and 80.3% of cases, respectively. The proportion of scores above 1.7 (defined as acceptable identification) were comparable for both extraction protocols using IDFP (
= 0.256). Moreover, rates of acceptable identification after DT performed on IDFP (93.9%) were significantly higher than those obtained after EA extraction with SAB (69.7%) or CAN2 (71.2%) (
= <0.001 and
= 0.001, respectively). Morphological aspects and antifungal susceptibility testing were similar between IDFP and SAB. IDFP is a culture plate that facilitates and improves the identification of filamentous fungi, allowing accurate routine identification of molds with MALDI-TOF-MS using a rapid-extraction protocol.</description><subject>Life Sciences</subject><subject>Microbiology and Parasitology</subject><subject>Mycology</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpdkd9r2zAQx8VoadOub3seetxg7u4ky5ZeBlnSHxkJLaWDvQlFllsV28osO5D_vk7Thq3cw8Hd5753x5eQTwjniEx-f7L1OWAm8wTVBzJCUDLJMvhzQEYASiSIPD8mJzE-AWCaCnFEjjkDgSxjI3J3sTZVbzofGhpKOpvSy7558PS2Mp2LdOEK39e0DC2dFa7pfOntHl6Eqoh0uaGL8Xw6oz996DYr134kh6Wpojt7zafk9-XF_eQ6md9czSbjeWJTmXZJyU2ONuVKSGbdEByskcIaw6QTjNki44AKleBLW-a5LRQM5yshGHDBBD8lP3a6q35Zu8IO57Wm0qvW16bd6GC8_r_T-Ef9ENY6Rwks3Qp83Qk8vhu7Hs_1tgY8B45CrnFgv7wua8Pf3sVO1z5aV1WmcaGPmnGR5jxDlQ7otx1q2xBj68q9NoLeWqZ_TRb6xTKNasA___vGHn7ziD8D4Q6P7g</recordid><startdate>20200423</startdate><enddate>20200423</enddate><creator>Robert, Marie Gladys</creator><creator>Romero, Charlotte</creator><creator>Dard, Céline</creator><creator>Garnaud, Cécile</creator><creator>Cognet, Odile</creator><creator>Girard, Thomas</creator><creator>Rasamoelina, Tahinamandranto</creator><creator>Cornet, Muriel</creator><creator>Maubon, Danièle</creator><general>American Society for Microbiology</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-4155-3675</orcidid></search><sort><creationdate>20200423</creationdate><title>Evaluation of ID Fungi Plates Medium for Identification of Molds by MALDI Biotyper</title><author>Robert, Marie Gladys ; Romero, Charlotte ; Dard, Céline ; Garnaud, Cécile ; Cognet, Odile ; Girard, Thomas ; Rasamoelina, Tahinamandranto ; Cornet, Muriel ; Maubon, Danièle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-f3a71c439582cecec30ca85caa28e522cd630191953bcf77cd904459552035253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Life Sciences</topic><topic>Microbiology and Parasitology</topic><topic>Mycology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Robert, Marie Gladys</creatorcontrib><creatorcontrib>Romero, Charlotte</creatorcontrib><creatorcontrib>Dard, Céline</creatorcontrib><creatorcontrib>Garnaud, Cécile</creatorcontrib><creatorcontrib>Cognet, Odile</creatorcontrib><creatorcontrib>Girard, Thomas</creatorcontrib><creatorcontrib>Rasamoelina, Tahinamandranto</creatorcontrib><creatorcontrib>Cornet, Muriel</creatorcontrib><creatorcontrib>Maubon, Danièle</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Robert, Marie Gladys</au><au>Romero, Charlotte</au><au>Dard, Céline</au><au>Garnaud, Cécile</au><au>Cognet, Odile</au><au>Girard, Thomas</au><au>Rasamoelina, Tahinamandranto</au><au>Cornet, Muriel</au><au>Maubon, Danièle</au><au>Hanson, Kimberly E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of ID Fungi Plates Medium for Identification of Molds by MALDI Biotyper</atitle><jtitle>Journal of clinical microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2020-04-23</date><risdate>2020</risdate><volume>58</volume><issue>5</issue><issn>0095-1137</issn><eissn>1098-660X</eissn><abstract>MALDI-TOF mass spectrometry (MS) identification of pathogenic filamentous fungi is often impaired by difficulties in harvesting hyphae embedded in the medium and long extraction protocols. The ID Fungi Plate (IDFP) is a novel culture method developed to address such difficulties and improve the identification of filamentous fungi by MALDI-TOF MS. We cultured 64 strains and 11 clinical samples on IDFP, Sabouraud agar-chloramphenicol (SAB), and ChromID Candida agar (CAN2). We then compared the three media for growth, ease of harvest, amount of material picked, and MALDI-TOF identification scores after either rapid direct transfer (DT) or a long ethanol-acetonitrile (EA) extraction protocol. Antifungal susceptibility testing and microscopic morphology after subculture on SAB and IDFP were also compared for ten molds. Growth rates and morphological aspects were similar for the three media. With IDFP, harvesting of fungal material for the extraction procedure was rapid and easy in 92.4% of cases, whereas it was tedious on SAB or CAN2 in 65.2% and 80.3% of cases, respectively. The proportion of scores above 1.7 (defined as acceptable identification) were comparable for both extraction protocols using IDFP (
= 0.256). Moreover, rates of acceptable identification after DT performed on IDFP (93.9%) were significantly higher than those obtained after EA extraction with SAB (69.7%) or CAN2 (71.2%) (
= <0.001 and
= 0.001, respectively). Morphological aspects and antifungal susceptibility testing were similar between IDFP and SAB. IDFP is a culture plate that facilitates and improves the identification of filamentous fungi, allowing accurate routine identification of molds with MALDI-TOF-MS using a rapid-extraction protocol.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>32051262</pmid><doi>10.1128/jcm.01687-19</doi><orcidid>https://orcid.org/0000-0003-4155-3675</orcidid><oa>free_for_read</oa></addata></record> |
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title | Evaluation of ID Fungi Plates Medium for Identification of Molds by MALDI Biotyper |
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