Dose‐dependent Inhibition of Gynecophoral Canal Protein Gene Expression in Vitro in the Schistosome (Schistosoma japonicum) by RNA Interference

The gynecophoral canal protein gene SjGCP of Schistosoma japonicum that is necessary for the pairing between the male and female worms is specifically expressed in the adult male worm. This protein is widely distributed in the adult female worm after pairing. Reverse transcription‐polymerase chain r...

Full description

Saved in:
Bibliographic Details
Published in:Acta biochimica et biophysica Sinica 2005-06, Vol.37 (6), p.386-390
Main Authors: CHENG, Guo‐Feng, LIN, Jiao‐Jiao, SHI, Yi, JIN, You‐Xin, FU, Zhi‐Qiang, JIN, Ya‐Mei, ZHOU, Yuan‐Cong, CAI, You‐Min
Format: Article
Language:English
Subjects:
Animals
dosage
Female
Fluorescent Antibody Technique
Gene Expression Regulation
Glycoproteins - biosynthesis
gynecophoral canal protein gene
Helminth Proteins - biosynthesis
Male
Research Papers
Reverse Transcriptase Polymerase Chain Reaction
RNA Interference
RNA, Double-Stranded - pharmacology
Schistosoma japonicum
Schistosoma japonicum - genetics
Schistosoma japonicum - metabolism
Sexual Behavior, Animal
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The gynecophoral canal protein gene SjGCP of Schistosoma japonicum that is necessary for the pairing between the male and female worms is specifically expressed in the adult male worm. This protein is widely distributed in the adult female worm after pairing. Reverse transcription‐polymerase chain reaction (RT‐PCR) and immunofluorescence were employed to analyze the relationship between the RNAi effect and dsRNA dosage in the parasites. The results revealed that the inhibition of SjGCP expression by siRNA is dose‐dependent. RT‐PCR analysis showed that the SjGCP transcript level was reduced by 75% when 100 nM dsRNA was applied. Edited by: Wang‐Yi LIU
ISSN:1672-9145
1745-7270
DOI:10.1111/j.1745-7270.2005.00058.x