Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards

In a preliminary clinical study, we observed that the combination of hydroxychloroquine and azithromycin was effective against SARS-CoV-2 by shortening the duration of viral load in Covid-19 patients. It is of paramount importance to define when a treated patient can be considered as no longer conta...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2020-06, Vol.39 (6), p.1059-1061
Main Authors: La Scola, Bernard, Le Bideau, Marion, Andreani, Julien, Hoang, Van Thuan, Grimaldier, Clio, Colson, Philippe, Gautret, Philippe, Raoult, Didier
Format: Article
Language:English
Subjects:
Animals
Azithromycin
Bacteriology
Betacoronavirus - isolation & purification
Betacoronavirus - physiology
Biomedical and Life Sciences
Biomedicine
Biotechnology
Brief Report
Cardiology and cardiovascular system
Cell culture
Chlorocebus aethiops
Coronavirus Infections - diagnosis
Coronavirus Infections - virology
COVID-19
E protein
Emerging diseases
France
Human health and pathology
Humans
Hydroxychloroquine
Infectious diseases
Internal Medicine
Life Sciences
Medical Microbiology
Microbiology and Parasitology
Pandemics
Parasitology
Patient Discharge
Patients
Pneumonia, Viral - diagnosis
Pneumonia, Viral - virology
Polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA, Viral - analysis
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
Specimen Handling - standards
Vero Cells
Viral diseases
Viral Load
Virology
Viruses
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Summary:In a preliminary clinical study, we observed that the combination of hydroxychloroquine and azithromycin was effective against SARS-CoV-2 by shortening the duration of viral load in Covid-19 patients. It is of paramount importance to define when a treated patient can be considered as no longer contagious. Correlation between successful isolation of virus in cell culture and Ct value of quantitative RT-PCR targeting E gene suggests that patients with Ct above 33–34 using our RT-PCR system are not contagious and thus can be discharged from hospital care or strict confinement for non-hospitalized patients.
ISSN:0934-9723
1435-4373
1435-4373
DOI:10.1007/s10096-020-03913-9