Orthogonal test design for the optimization of superparamagnetic chitosan plasmid gelatin microspheres that promote vascularization of artificial bone

The optimal conditions for the preparation of superparamagnetic chitosan plasmid (pReceiver‐M29‐VEGF165/DH5a) gelatin microspheres (SPCPGMs) were determined. Then, the performance of the SPCPGMs during neovascularization was evaluated in vivo. The SPCPGMs were prepared through a cross‐linking curing...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2020-05, Vol.108 (4), p.1439-1449
Main Authors: Tao, Chen, Lina, Xie, Changxuan, Wang, Cong, Luo, Xiaolan, Yang, Tao, Huang, Hong, An
Format: Article
Language:English
Subjects:
Angiogenesis
Biomedical materials
Chitosan
Design optimization
Emulsification
Fabrication
Gelatin
Glutaraldehyde
In vivo methods and tests
Iron oxides
magnetic field
Magnetic fields
magnetic gene‐loaded microspheres
Materials research
Materials science
Microspheres
Nanoparticles
Original Research Report
Original Research Reports
Plasmids
Tissue engineering
Vascularization
VEGF
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Summary:The optimal conditions for the preparation of superparamagnetic chitosan plasmid (pReceiver‐M29‐VEGF165/DH5a) gelatin microspheres (SPCPGMs) were determined. Then, the performance of the SPCPGMs during neovascularization was evaluated in vivo. The SPCPGMs were prepared through a cross‐linking curing method and then filled into the hollow scaffold of an artificial bone. Neovascularization at the bone defect position was histologically examined in samples collected 2, 4, 6, and 8 weeks after the operation. The cellular magnetofection rate of superparamagnetic chitosan nanoparticles/plasmid (pReceiver‐M29‐VEGF165/DH5a) complexes reached 1–3% under static magnetic field (SMF). Meanwhile, the optimal conditions for SPCPGM fabrication were 20% Fe3O4 (w/v), 4 mg of plasmid, 5.3 mg of glutaraldehyde, and 500 rpm of emulsification rotate speed. Under oscillating magnetic fields (OMFs), 4–6 μg of plasmids was released daily for 21 days. Under the combined application of SMF and OMF, evident neovascularization occurred at the bone defect position 6 weeks after the operation. This result is expected to provide a new type of angiogenesis strategy for the research of bone tissue engineering.
ISSN:1552-4973
1552-4981
DOI:10.1002/jbm.b.34491