miR-338-5p Targets Epidermal Growth Factor-Containing Fibulin-Like Extracellular Matrix Protein 1 to Inhibit the Growth and Invasion of Trophoblast Cells in Selective Intrauterine Growth Restriction

Selective intrauterine growth restriction (sIUGR) is a disorder of monochorionic (MC) twin pregnancies. However, the underlying mechanism remains largely unknown. Trophoblast cells are the major component of the placenta. Dysfunction of trophoblast cells is associated with placental dysfunction. Our...

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Bibliographic Details
Published in:Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2020-06, Vol.27 (6), p.1357-1364
Main Authors: Wen, Hong, Hu, Ying, Chen, Lu, Zhao, Li, Yang, Xinyun
Format: Article
Language:English
Subjects:
Apoptosis - physiology
Cell Line
Cell Movement - physiology
Cell Proliferation - physiology
Embryology
Extracellular Matrix Proteins - metabolism
Female
Fetal Growth Retardation - metabolism
Fetal Growth Retardation - pathology
Humans
Medicine
Medicine & Public Health
MicroRNAs - metabolism
Obstetrics/Perinatology/Midwifery
Original
Original Article
Phosphorylation
Placenta - metabolism
Placenta - pathology
Pregnancy
Reproductive Medicine
Trophoblasts - metabolism
Trophoblasts - pathology
Up-Regulation
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Summary:Selective intrauterine growth restriction (sIUGR) is a disorder of monochorionic (MC) twin pregnancies. However, the underlying mechanism remains largely unknown. Trophoblast cells are the major component of the placenta. Dysfunction of trophoblast cells is associated with placental dysfunction. Our previous study identified miR-338-5p is downregulated in placenta tissues sharing larger twins of sIUGR. In the present study, we aimed to investigate the role of miR-338-5p in trophoblast cells and explored its target. Our results further indicated that miR-338-5p was downregulated in placental tissues supporting larger twins of sIUGR, whereas epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1) was upregulated. Moreover, miR-338-5p overexpression suppressed the growth and invasion of trophoblast cells. Importantly, results from luciferase reporter assay demonstrated that miR-338-5p bound on the 3′-UTR of EFEMP1. miR-338-5p suppressed the growth and invasion of trophoblast cells via targeting EFEMP1. Further, miR-338-5p/EFEMP1 might disrupt the function of trophoblast cells via inhibiting the phosphorylation of AKT.
ISSN:1933-7191
1933-7205
DOI:10.1007/s43032-020-00160-3