Spatial Sequencing: A Perspective

Fluorescent spatial sequencing brings next-generation sequencing into a new realm capable of identifying nucleic acids in the cell's natural environment. For the first time, scientists are able to multiplex the assignment of specific locations to hundreds of transcriptional targets and lay the...

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Published in:Journal of biomolecular techniques 2020-07, Vol.31 (2), p.44-46
Main Authors: Turczyk, Brian M, Busby, Michele, Martin, Allison L, Daugharthy, Evan R, Myung, Daniel, Terry, Richard C, Inverso, Samuel A, Kohman, Richie E, Church, George M
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container_end_page 46
container_issue 2
container_start_page 44
container_title Journal of biomolecular techniques
container_volume 31
creator Turczyk, Brian M
Busby, Michele
Martin, Allison L
Daugharthy, Evan R
Myung, Daniel
Terry, Richard C
Inverso, Samuel A
Kohman, Richie E
Church, George M
description Fluorescent spatial sequencing brings next-generation sequencing into a new realm capable of identifying nucleic acids in the cell's natural environment. For the first time, scientists are able to multiplex the assignment of specific locations to hundreds of transcriptional targets and lay the foundation for understanding how genetic changes control the fate of each cell within the tissue microenvironment. In this perspective, we discuss the capabilities of fluorescent spatial sequencing in the context of other spatial imaging technologies and describe how these new technologies offer a data-rich, multiomic solution to many research applications. Fluorescent spatial sequencing has opened options for exploring many fundamental questions in biology, helping us gain a better understanding of cell and tissue development and disease progression.
doi_str_mv 10.7171/jbt.20-3102-003
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title Spatial Sequencing: A Perspective
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