A GoldenBraid cloning system for synthetic biology in social amoebae

GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present Gol...

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Bibliographic Details
Published in:Nucleic acids research 2020-05, Vol.48 (8), p.4139-4146
Main Authors: Kundert, Peter, Sarrion-Perdigones, Alejandro, Gonzalez, Yezabel, Katoh-Kurasawa, Mariko, Hirose, Shigenori, Lehmann, Peter, Venken, Koen J T, Shaulsky, Gad
Format: Article
Language:English
Subjects:
Data Resources and Analyses
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Summary:GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkaa185