Plasmacytoid dendritic cell deficiency in neonates enhances allergic airway inflammation via reduced production of IFN-α

• Published in: Cellular & molecular immunology 2020-05, Vol.17 (5), p.519-532
• Main Authors: Wu, Min, Gao, Liuchuang, He, Miao, Liu, Hangyu, Jiang, Han, Shi, Ketai, Shang, Runshi, Liu, Bing, Gao, Shan, Chen, Hebin, Gong, Feili, Gelfand, Erwin W., Huang, Yafei, Han, Junyan
• Format: Article
• Language: English
• Subjects: 631/250/2152; 631/250/262; 631/250/347; Adoptive transfer; Allergens; Allergic diseases; Antibodies; Asthma; Biomedical and Life Sciences; Biomedicine; CCL20 protein; Cdc2 protein; Dendritic cells; Epithelial cells; Granulocyte-macrophage colony-stimulating factor; Helper cells; Hypersensitivity; Immunology; Inflammation; Lymphocytes T; Medical Microbiology; Microbiology; Neonates; Respiratory tract diseases; Rodents; Vaccine; α-Interferon
• ISSN: 1672-7681; 2042-0226
• DOI: 10.1038/s41423-019-0333-y

Allergic asthma, a chronic inflammatory airway disease associated with type 2 cytokines, often originates in early life. Immune responses at an early age exhibit a Th2 cell bias, but the precise mechanisms remain elusive. Plasmacytoid dendritic cells (pDCs), which play a regulatory role in allergic asthma, were shown to be deficient in neonatal mice. We report here that this pDC deficiency renders neonatal mice more susceptible to severe allergic airway inflammation than adult mice in an OVA-induced experimental asthma model. Adoptive transfer of pDCs or administration of IFN-α to neonatal mice prevented the development of allergic inflammation in wild type but not in IFNAR1 −/− mice. Similarly, adult mice developed more severe allergic inflammation when pDCs were depleted. The protective effects of pDCs were mediated by the pDC-/IFN-α-mediated negative regulation of the secretion of epithelial cell-derived CCL20, GM-CSF, and IL-33, which in turn impaired the recruitment of cDC2 and ILC2 cells to the airway. In asthmatic patients, the percentage of pDCs and the level of IFN-α were lower in children than in adults. These results indicate that impairment of pDC-epithelial cell crosstalk in neonates is a susceptibility factor for the development of allergen-induced allergic airway inflammation.