Identification of B-cell epitopes on the betanodavirus capsid protein

The pepscan procedure was used to identify betanodavirus B-cell epitopes recognized by neutralizing mouse monoclonal antibodies (MAbs) and serum samples obtained from sea bass, Dicentrarchus labrax, naturally infected with betanodavirus. Pepscan was performed with a panel of thirty-four 12-mer synth...

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Bibliographic Details
Published in:Journal of fish diseases 2007-07, Vol.30 (7), p.419-426
Main Authors: Costa, J.Z, Adams, A, Bron, J.E, Thompson, K.D, Starkey, W.G, Richards, R.H
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies, Monoclonal - immunology
Bass - blood
Bass - virology
Betanodavirus
Capsid Proteins - chemistry
Capsid Proteins - immunology
Dicentrarchus labrax
Epitope Mapping
Epitopes, B-Lymphocyte - chemistry
Epitopes, B-Lymphocyte - immunology
luminex
Marine
Mice
Mice, Inbred BALB C
monoclonal antibodies
Nodaviridae - chemistry
Nodaviridae - immunology
Nodavirus
Original
pepscan
xMAP technology
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Summary:The pepscan procedure was used to identify betanodavirus B-cell epitopes recognized by neutralizing mouse monoclonal antibodies (MAbs) and serum samples obtained from sea bass, Dicentrarchus labrax, naturally infected with betanodavirus. Pepscan was performed with a panel of thirty-four 12-mer synthetic peptides that mimicked the entire betanodavirus capsid protein. Sea bass serum samples reacted strongly with three regions of the capsid protein comprising amino acid residues 1-32, 91-162 and 181-212. The latter region was also recognized by neutralizing MAbs and coincided with a region of high antigenic propensity identified by an antigen prediction algorithm. These data suggest that a region of the betanodavirus capsid protein spanning amino acid residues 181-212 may represent a neutralization domain that could potentially be used to inform the development of nodavirus vaccines and immunodiagnostic reagents.
ISSN:0140-7775
1365-2761
DOI:10.1111/j.1365-2761.2007.00824.x