Intraoperative Laser Speckle Contrast Imaging For Real-Time Visualization of Cerebral Blood Flow in Cerebrovascular Surgery: Results From Pre-Clinical Studies

Cerebrovascular surgery can benefit from an intraoperative system that conducts continuous monitoring of cerebral blood flow (CBF). Such a system must be handy, non-invasive, and directly integrated into the surgical workflow. None of the currently available techniques, considered alone, meets all t...

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Bibliographic Details
Published in:Scientific reports 2020-05, Vol.10 (1), p.7614-7614, Article 7614
Main Authors: Mangraviti, Antonella, Volpin, Francesco, Cha, Jaepyeong, Cunningham, Samantha I., Raje, Karan, Brooke, M. Jason, Brem, Henry, Olivi, Alessandro, Huang, Judy, Tyler, Betty M., Rege, Abhishek
Format: Article
Language:English
Subjects:
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Angiography
Animal models
Animals
Blood flow
Cerebral blood flow
Cerebrovascular Circulation
Femoral artery
Humanities and Social Sciences
Ischemia
Lasers
Microvasculature
Molecular Imaging - instrumentation
Monitoring, Intraoperative - instrumentation
multidisciplinary
Perfusion
Rats
Science
Science (multidisciplinary)
Surgeons
Surgery
Time Factors
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Summary:Cerebrovascular surgery can benefit from an intraoperative system that conducts continuous monitoring of cerebral blood flow (CBF). Such a system must be handy, non-invasive, and directly integrated into the surgical workflow. None of the currently available techniques, considered alone, meets all these criteria. Here, we introduce the SurgeON™ system: a newly developed non-invasive modular tool which transmits high-resolution Laser Speckle Contrast Imaging (LSCI) directly onto the eyepiece of the surgical microscope. In preclinical rodent and rabbit models, we show that this system enabled the detection of acute perfusion changes as well as the recording of temporal response patterns and degrees of flow changes in various microvascular settings, such as middle cerebral artery occlusion, femoral artery clipping, and complete or incomplete cortical vessel cautery. During these procedures, a real-time visualization of vasculature and CBF was available in high spatial resolution through the eyepiece as a direct overlay on the live morphological view of the surgical field. Upon comparison with indocyanine green angiography videoangiography (ICG-VA) imaging, also operable via SurgeON, we found that direct-LSCI can produce greater information than ICG-VA and that continuous display of data is advantageous for performing immediate LSCI-guided adjustments in real time.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-64492-5