SARS-CoV-2 detection by direct rRT-PCR without RNA extraction

Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar® SARS-CoV-2 rRT-PCR kit (Altona Diagnostics). F...

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Bibliographic Details
Published in:Journal of clinical virology 2020-07, Vol.128, p.104423-104423, Article 104423
Main Authors: Merindol, Natacha, Pépin, Geneviève, Marchand, Caroline, Rheault, Marylène, Peterson, Christine, Poirier, André, Houle, Claudia, Germain, Hugo, Danylo, Alexis
Format: Article
Language:English
Subjects:
Betacoronavirus - genetics
Betacoronavirus - isolation & purification
Coronavirus
Coronavirus Infections - diagnosis
Coronavirus Infections - virology
COVID-19
COVID19
Direct rRTPCR
Humans
Nasopharynx - virology
Pandemics
Pneumonia, Viral - diagnosis
Pneumonia, Viral - virology
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA extraction
RNA, Viral - genetics
SARS-CoV-2
Specimen Handling
Virus detection
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Summary:Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar® SARS-CoV-2 rRT-PCR kit (Altona Diagnostics). Furthermore, we assessed the impact of swab storage media composition on PCR efficiency. We show that SeeGene and Altona’s assays provide similar efficiency. Importantly, we provide evidence that RNA extraction can be successfully bypassed when samples are stored in UTM medium or in molecular water but not when samples are stored in saline solution and in Hanks medium.
ISSN:1386-6532
1873-5967
DOI:10.1016/j.jcv.2020.104423