Satb2 is required for the regionalization of retrosplenial cortex

The retrosplenial cortex (Rsp) is a transitional cortex located between the neocortex and archicortex, but the molecular mechanism specifying Rsp from the archicortex remains elusive. We here report that the transcription factor Satb2 is required for specifying Rsp identity during its morphogenesis....

Full description

Saved in:
Bibliographic Details
Published in:Cell death and differentiation 2020-05, Vol.27 (5), p.1604-1617
Main Authors: Zhang, Lei, Song, Ning-Ning, Zhang, Qiong, Mei, Wan-Ying, He, Chun-Hui, Ma, Pengcheng, Huang, Ying, Chen, Jia-Yin, Mao, Bingyu, Lang, Bing, Ding, Yu-Qiang
Format: Article
Language:English
Subjects:
13/51
13/89
42/109
631/208/135
631/378/2611
64/60
Animal memory
Animals
Apoptosis
Archicortex
Biochemistry
Biomedical and Life Sciences
Cell Adhesion Molecules, Neuronal - metabolism
Cell Biology
Cell Cycle Analysis
Cell Lineage
Cerebral cortex
Gene Expression Regulation
Gyrus Cinguli - metabolism
HEK293 Cells
Humans
Life Sciences
Mice, Knockout
Morphogenesis
Neocortex
Nuclear Receptor Subfamily 4, Group A, Member 2 - metabolism
Repressor Proteins - metabolism
Stem Cells
Subiculum
Tumor Suppressor Proteins - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The retrosplenial cortex (Rsp) is a transitional cortex located between the neocortex and archicortex, but the molecular mechanism specifying Rsp from the archicortex remains elusive. We here report that the transcription factor Satb2 is required for specifying Rsp identity during its morphogenesis. In Satb2 CKO mice, the boundary between the Rsp and archicortex [i.e., subiculum (SubC)] disappears as early as E17.5, and Rsp efferent projection is aberrant. Rsp-specific genes are lost, whereas SubC-specific genes are ectopically expressed in Rsp of Satb2 CKO mice. Furthermore, cell-autonomous role of Satb2 in maintaining Rsp neuron identity is revealed by inactivation of Satb2 in Rsp neurons. Finally, Satb2 represses the transcription of Nr4a2. The misexpression of Nr4a2 together with Ctip2 induces expression of SubC-specific genes in wild-type Rsp, and simultaneous knockdown of these two genes in Rsp Satb2-mutant cells prevents their fate transition to SubC identity. Thus, Satb2 serves as a determinant gene in the Rsp regionalization by repressing Nr4a2 and Ctip2 during cortical development.
ISSN:1350-9047
1476-5403
1476-5403
DOI:10.1038/s41418-019-0443-1