Potency of Oral Rehydration Solution in Inducing Fluid Absorption is Related to Glucose Concentration

• Published in: Scientific reports 2020-05, Vol.10 (1), p.7803, Article 7803
• Main Authors: Buccigrossi, Vittoria, Lo Vecchio, Andrea, Bruzzese, Eugenia, Russo, Carla, Marano, Antonella, Terranova, Sara, Cioffi, Valentina, Guarino, Alfredo
• Format: Article
• Language: English
• Subjects: 631/443; 692/4020; 9/30; Absorption; Bicarbonates - pharmacology; Caco-2 Cells; Child; Children; Clinical trials; Diarrhea; Diarrhea - metabolism; Diarrhea - prevention & control; Diarrhea - therapy; Electrolyte balance; Fluid Therapy - methods; Gastroenteritis; Gastroenterology; Glucose; Glucose - metabolism; Glucose - pharmacology; Glucose transporter; Humanities and Social Sciences; Humans; Intestinal Absorption - drug effects; Intestine; multidisciplinary; Osmolar Concentration; Palatability; Potassium - metabolism; Potassium Chloride - pharmacology; Rehydration; Rehydration Solutions - pharmacology; Rotavirus; Saline Solution - pharmacology; Science; Science (multidisciplinary); Secretion; Sodium; Sodium - metabolism; Sodium Chloride - pharmacology; Sodium-glucose cotransporter; Sodium-Glucose Transporter 1 - genetics; Titration; Water-Electrolyte Balance - drug effects
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-64818-3

Oral rehydration solutions (ORSs) is the key treatment of acute diarrhea in children, as it restores the electrolyte balance by stimulating the intestinal sodium/glucose transporter SGLT1 to induce fluid absorption. The World Health Organization (WHO) and The European Society for Paediatric Gastroenterology Hepatology and Nutrition (ESPGHAN) proposed ORSs with different chemical compositions. The main agent of childhood acute gastroenteritis is rotavirus (RV). We evaluate the effects of ORS with different concentration of glucose and sodium on RV induced secretion. Ussing chambers technique was used for electophysiology experiments to evaluate ion fluid flux. ESPGHAN ORS (sodium 60 mmol/L and glucose 111 mmol/L) induced a more potent proabsorptive effect in Caco-2 cells than WHO ORS, and this effect depended on the sodium/glucose ratio. Titration experiments showed that RV-induced fluid secretion can be reverted to a proabsorptive direction when sodium and glucose concentration fall in specific ranges, specifically 45–60 mEq/L and 80–110 mM respectively. The results were confirmed by testing commercial ORSs. These findings indicated that ORS proabsorptive potency depends on sodium and glucose concentrations. Optimal ORS composition should be tailored to reduce RV-induced ion secretion by also considering palatability. These in vitro data should be confirmed by clinical trials.