The contribution of cis- and trans-acting variants to gene regulation in wild and domesticated barley under cold stress and control conditions

Barley, like other crops, has experienced a series of genetic changes that have impacted its architecture and growth habit to suit the needs of humans, termed the domestication syndrome. Domestication also resulted in a concomitant bottleneck that reduced sequence diversity in genes and regulatory r...

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Bibliographic Details
Published in:Journal of experimental botany 2020-05, Vol.71 (9), p.2573-2584
Main Authors: Haas, Matthew, Himmelbach, Axel, Mascher, Martin
Format: Article
Language:English
Subjects:
Alleles
Cold-Shock Response - genetics
Crops, Agricultural - genetics
Domestication
Hordeum - genetics
Research Papers
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Summary:Barley, like other crops, has experienced a series of genetic changes that have impacted its architecture and growth habit to suit the needs of humans, termed the domestication syndrome. Domestication also resulted in a concomitant bottleneck that reduced sequence diversity in genes and regulatory regions. Little is known about regulatory changes resulting from domestication in barley. We used RNA sequencing to examine allele-specific expression in hybrids between wild and domesticated barley. Our results show that most genes have conserved regulation. In contrast to studies of allele-specific expression in interspecific hybrids, we find almost a complete absence of trans effects. We also find that cis regulation is largely stable in response to short-term cold stress. Our study has practical implications for crop improvement using wild relatives. Genes regulated in cis are more likely to be expressed in a new genetic background at the same level as in their native background.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/eraa036