Macrophage-like THP-1 cells show effective uptake of silica nanoparticles carrying inactivated diphtheria toxoid for vaccination

Nanoparticles may be used in vaccinology as an antigen delivery and/or an immunostimulant to enhance immunity. Porous silica has been identified as an effective adjuvant for more than a decade, and we have therefore investigated the take up rate by an immortalized macrophage-like cell line of a numb...

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Bibliographic Details
Published in:Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology 2020, Vol.22 (1), p.23-23, Article 23
Main Authors: Huang, Xinyue, Cavalcante, Danielle Paixão, Townley, Helen E
Format: Article
Language:English
Subjects:
Antigens
Biocompatibility
Cell viability
Characterization and Evaluation of Materials
Chemical synthesis
Chemistry and Materials Science
Diphtheria
Immunostimulants
Inorganic Chemistry
Internalization
Lasers
Macrophages
Materials Science
Nanoparticles
Nanotechnology
Optical Devices
Optics
Photonics
Physical Chemistry
Pore size
Pores
Porosity
Research Paper
Silica
Silicon dioxide
Sol-gel processes
Vaccination
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Summary:Nanoparticles may be used in vaccinology as an antigen delivery and/or an immunostimulant to enhance immunity. Porous silica has been identified as an effective adjuvant for more than a decade, and we have therefore investigated the take up rate by an immortalized macrophage-like cell line of a number of mesoporous silica nanoparticles (MSNPs) with differing diameter and pore size. The MSNPs were synthesized using a sol-gel reaction and post-synthesis removal of the template. The MSNPs showed a clear distribution in take up rate peaking at 217 nm, whereas a comparison with solid spherical nanoparticles showed a similar distribution peaking at 377 nm. The MSNPs were investigated before and after loading with antigen. Diphtheria toxoid was used as a proof-of-concept antigen and showed a peak macrophage internalization of 53.42% for loaded LP3 particles which had a diameter of 217.75 ± 5.44 nm and large 16.5 nm pores. Optimal MSNP sizes appeared to be in the 200–400 nm range, and larger pores showed better antigen loading. The mesoporous silica particles were shown to be generally biocompatible, and cell viability was not altered by the loading of particles with or without antigen. Graphical abstract
ISSN:1388-0764
1572-896X
DOI:10.1007/s11051-019-4720-1