Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil

Purpose The aim of the present study was to detect and genotype T. gondii in free-range chickens destined to human consumption in Alagoas state, Brazil. Methods Two hundred blood samples were collected from free-range chickens and submitted to indirect immunofluorescence antibody test (IFAT). Brain...

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Bibliographic Details
Published in:Acta parasitologica 2020-09, Vol.65 (3), p.774-778
Main Authors: dos Santos Silva, Ana Clécia, de Barros, Luiz Daniel, Barros, Viviane Melo Coelho, de Alcântara, Adrianne Mota, Andrade, Müller Ribeiro, Garcia, João Luis, Mota, Rinaldo Aparecido, Porto, Wagnner José Nascimento
Format: Article
Language:English
Subjects:
Animal Systematics/Taxonomy/Biogeography
Animal tissues
Animals
Antibodies
Antibodies, Protozoan - blood
Bioassays
Biological Assay
Biomedical and Life Sciences
Biomedicine
Blood
Brazil - epidemiology
Chickens
Chickens - parasitology
Consumption
DNA, Protozoan - genetics
Ecology
Food Microbiology
Genetic diversity
Genetic Variation
Genotype
Genotype & phenotype
Genotypes
Humans
Immunofluorescence
Medical Microbiology
Mice
Microbiology
Parasites
Parasitology
Poultry
Poultry - parasitology
Poultry Diseases - epidemiology
Poultry Diseases - parasitology
Protozoa
Short Communication
Toxoplasma - classification
Toxoplasma - genetics
Toxoplasmosis, Animal - epidemiology
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Summary:Purpose The aim of the present study was to detect and genotype T. gondii in free-range chickens destined to human consumption in Alagoas state, Brazil. Methods Two hundred blood samples were collected from free-range chickens and submitted to indirect immunofluorescence antibody test (IFAT). Brain tissue from 14 animals randomly selected were subjected to mouse bioassay. Positive samples in mouse bioassay were submitted to PCR and genotyped by PCR–RFLP. Results Out of two hundred blood samples from chickens, 72 (36%) samples were considered positive by IFAT. Two T. gondii strains were isolated, both being characterized as atypical and classified as #146 and a new genotype, named #279 in ToxoDB database. Conclusions Our results showed a sero-occurrence of T. gondii in free-range chickens intended for humans, and the genetic diversity of the parasite in Brazil, with a new genotype described.
ISSN:1230-2821
1896-1851
DOI:10.2478/s11686-020-00194-2