Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt

• Published in: Turkish journal of pharmaceutical sciences 2020-04, Vol.17 (2), p.127-135
• Main Authors: Nabil-Adam, Asmaa, Shreadah, Mohamed A, El Moneam, Nehad M Abd, El-Assar, Samy A
• Format: Article
• Language: English
• Subjects: Eczacılık; Original; Sağlık Hizmetleri; Tıp
• ISSN: 1304-530X; 2148-6247
• DOI: 10.4274/tjps.galenos.2018.72677

The present study revealed the presence of bioactive constituents in sponge (HES) extract collected from the Red Sea using skin and scuba diving. Cytotoxicity was tested against hepatocellular carcinoma cell lines as a prescreening test. The HES extract had high contents of total phenolic compounds (0.061 mg/g), flavonoids (0.2839 mg/g), and carotenoids (1.976 mg/g). Moreover, the HES extract showed high antioxidant capacity with 93.0% and 99% at 1 mg using 2.2'-Diphenyl-α-picrylhydrazyl and 2.2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), respectively. Cytotoxic activity against cancerous cell lines showed that the HES extract could inhibit cell growth effectively with IC =47.5 μg/mL. Furthermore, anticancer activity using protein tyrosine kinase and sphingosine kinase 1 inhibitor screening assays resulted in 71.66% and 85.21% inhibition activity, respectively. The anti-inflammatory assays showed that the inhibition activity against cyclooxygenase (COX ), COX , interleukin-6, and tumor necrosis factor-α was 71.82%, 81.13%, 80.89%, and 59.74%, respectively. At the same time, the anti-Alzheimer results using acetylcholine inhibition assay showed high activity at 1 mg with 83.51%. Additionally, the antiviral activity using the reverse transcriptase inhibition assay was 91.70%. This marine sponge isolated from the Red Sea showed tremendous activity against many diseases and it is considered an excellent source for bioactive pharmaceutical compounds.