Low temperature synergistically promotes wounding-induced indole accumulation by INDUCER OF CBF EXPRESSION-mediated alterations of jasmonic acid signaling in Camellia sinensis

• Published in: Journal of experimental botany 2020-03, Vol.71 (6), p.2172-2185
• Main Authors: Zhou, Ying, Zeng, Lanting, Hou, Xingliang, Liao, Yinyin, Yang, Ziyin
• Format: Article
• Language: English
• Subjects: Camellia sinensis; Cyclopentanes; Indoles; Oxylipins; Plant Proteins - genetics; Research Papers; Temperature
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/erz570

Abstract Plants have to cope with various environmental stress factors which significantly impact plant physiology and secondary metabolism. Individual stresses, such as low temperature, are known to activate plant volatile compounds as a defense. However, less is known about the effect of multiple stresses on plant volatile formation. Here, the effect of dual stresses (wounding and low temperature) on volatile compounds in tea (Camellia sinensis) plants and the underlying signalling mechanisms were investigated. Indole, an insect resistance volatile, was maintained at a higher content and for a longer time under dual stresses compared with wounding alone. CsMYC2a, a jasmonate (JA)-responsive transcription factor, was the major regulator of CsTSB2, a gene encoding a tryptophan synthase β-subunit essential for indole synthesis. During the recovery phase after tea wounding, low temperature helped to maintain a higher JA level. Further study showed that CsICE2 interacted directly with CsJAZ2 to relieve inhibition of CsMYC2a, thereby promoting JA biosynthesis and downstream expression of the responsive gene CsTSB2 ultimately enhancing indole biosynthesis. These findings shed light on the role of low temperature in promoting plant damage responses and advance knowledge of the molecular mechanisms by which multiple stresses coordinately regulate plant responses to the biotic and abiotic environment. INDUCER OF CBF EXPRESSION interacts with JASMONATE ZIM-DOMAIN proteins to relieve repression of MYC2 in tea plants, leading to enhanced accumulation of the insect resistance-associated volatile indole.