In Vitro Characterization of Adipose Stem Cells Non-Enzymatically Extracted from the Thigh and Abdomen

Autologous fat grafting is a surgical technique in which adipose tissue is transferred from one area of the body to another, in order to reconstruct or regenerate damaged or injured tissues. Before reinjection, adipose tissue needs to be purified from blood and cellular debris to avoid inflammation...

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Bibliographic Details
Published in:International journal of molecular sciences 2020-04, Vol.21 (9), p.3081
Main Authors: Dai Prè, Elena, Busato, Alice, Mannucci, Silvia, Vurro, Federica, De Francesco, Francesco, Riccio, Valentina, Solito, Samantha, Biswas, Reetuparna, Bernardi, Paolo, Riccio, Michele, Sbarbati, Andrea
Format: Article
Language:English
Subjects:
Abdomen
Adipose tissue
Adipose Tissue - cytology
Autografts
Biomarkers
Cell Differentiation
Cell number
Cell Separation
Cell Survival
Disaggregation
Fibroblasts
Growth rate
Humans
Immunophenotyping
Mesenchyme
Morphology
Purification
Reconstructive surgery
Reinjection
Stem cells
Stem Cells - cytology
Stem Cells - metabolism
Thigh
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Summary:Autologous fat grafting is a surgical technique in which adipose tissue is transferred from one area of the body to another, in order to reconstruct or regenerate damaged or injured tissues. Before reinjection, adipose tissue needs to be purified from blood and cellular debris to avoid inflammation and preserve the graft viability. To perform this purification, different enzymatic and mechanical methods can be used. In this study, we characterized in vitro the product of a closed automatic device based on mechanical disaggregation, named Rigenera , focusing on two sites of adipose tissue harvesting. At first, we optimized the Rigenera operating timing, demonstrating that 60 s of treatment allows a higher cellular yield, in terms of the cell number and growth rate. This result optimizes the mechanical disaggregation and it can increase the clinical efficiency of the final product. When comparing the extracted adipose samples from the thigh and abdomen, our results showed that the thigh provides a higher number of mesenchymal-like cells, with a faster replication rate and a higher ability to form colonies. We can conclude that by collecting adipose tissue from the thigh and treating it with the Rigenera device for 60 s, it is possible to obtain the most efficient product.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms21093081