Efficient silencing gene construct for resistance to multiple common bean (Phaseolus vulgaris L.) viruses

One promising strategy to engineer plants that are resistant to plant pathogens involves transforming plants with RNA silencing constructs for resistance to multiple pathogens. Garden bean is significantly damaged by bean common mosaic virus (BCMV), bean common mosaic necrosis virus (BCMNV) and cucu...

Full description

Saved in:
Bibliographic Details
Published in:3 Biotech 2020-06, Vol.10 (6), p.278-278, Article 278
Main Authors: Azizi, Abdolbaset, Verchot, Jeanmarie, Moieni, Ahmad, Shams-bakhsh, Masoud
Format: Article
Language:English
Subjects:
Accumulation
Agriculture
Agrobacterium
Antisense RNA
Apoptosis
Beans
Bioinformatics
Biomaterials
Biotechnology
Cancer Research
Chemistry
Chemistry and Materials Science
Efficiency
Genetic transformation
Necrosis
Original
Original Article
Pathogens
Phaseolus vulgaris
Ribonucleic acid
RNA
RNA-mediated interference
Signs and symptoms
Stem Cells
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:One promising strategy to engineer plants that are resistant to plant pathogens involves transforming plants with RNA silencing constructs for resistance to multiple pathogens. Garden bean is significantly damaged by bean common mosaic virus (BCMV), bean common mosaic necrosis virus (BCMNV) and cucumber mosaic virus (CMV). In this study, we prepared constructs producing sense, antisense and hairpin RNA (hpRNA) structures to target single as well as multiple viruses. Silencing efficiency of these constructions was analyzed using Agrobacterium (GV3101) transient expression in Nicothinia bethamiana and Phaseolus vulgaris plants. The results showed significantly reduced disease symptoms and virus accumulation in N. bethamiana plants. Generally, the efficiency of the prepared constructs was hairpin, antisense and sense, respectively, and also, there was a significant difference between mono-gene and multiple-gene constructs for reducng virus accumulation and the multiple-gene constructs showed higher effectiveness. Experiments in this study showed that using Agrobacterium harboring binary constructs containing a Caenorhabditis elegans gene, Ced-9 , or a plant gene, At Bag-4 , anti-apoptosis gene as a mix suspension with an Agrobacterium containing p FGC-BNC.h, a plasmid containing multiple gene fragments consisting of BCMV-CP, BCMNV-HC-Pro and CMV-2b, improved the efficiency of p FGC-BNC.h transformation. We showed reduced virus accumulation in these transgenic bean plans.
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-020-02276-4