Study on myelin injury of AD mice treated with Shenzhiling oral liquid in the PI3K/Akt–mTOR pathway

• Published in: International journal of immunopathology and pharmacology 2020, Vol.34, p.2058738420923907-2058738420923907
• Main Authors: Wang, Yahan, Chen, Fang, Wang, Pengwen, Mana, Lulu, Sheng, Ning, Huang, Shuaiyang
• Format: Article
• Language: English
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Alzheimer Disease - drug therapy; Alzheimer Disease - pathology; Alzheimer's disease; Animals; Cognition Disorders - prevention & control; Cognition Disorders - psychology; Disks Large Homolog 4 Protein - metabolism; Donepezil; Drug dosages; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - therapeutic use; Gene expression; Humans; Immunohistochemistry; Maze Learning; Medicine, Chinese Traditional; Mice; Mice, Inbred C57BL; Mice, Transgenic; Myelin; Myelin basic protein; Myelin Basic Protein - metabolism; Myelin Sheath - pathology; Neurodegenerative diseases; Oncogene Protein v-akt - drug effects; Original; Phosphatidylinositol 3-Kinases - drug effects; Polymerase chain reaction; Postsynaptic density; Postsynaptic density proteins; RNA-directed DNA polymerase; Signal Transduction - drug effects; TOR protein; TOR Serine-Threonine Kinases - drug effects; Traditional Chinese medicine; Transgenic mice
• ISSN: 2058-7384; 0394-6320; 2058-7384
• DOI: 10.1177/2058738420923907

Shenzhiling oral liquid (SZL) is a Traditional Chinese Medicine (TCM) compound to be approved by the China Food and Drug Administration (CFDA) (Z20120010) for the treatment of mild-to-moderate Alzheimer’s disease (AD). However, its mechanism in early AD is not clear. We studied its mechanism in protecting myelin. Three-month-old APPswe/PS1dE9double transgenic mice were used as AD model and wild-type C57BL/6 mice were used as control. After 3-month intervention, the Morris water maze was used to detect behavioural changes. Myelin mTOR pathway (PI3K, p-PI3K, Akt, p-Akt, mTOR, p-mTOR), myelin basic protein (MBP) and postsynaptic density protein 95 (PSD95) were detected by immunohistochemistry and western blot and reverse transcriptase polymerase chain reaction (RT-PCR). After 3 months of SZL treatment, compared with the model group (M), SZL medium-dose (SM) and SZL low-dose groups (SL) exhibited increased staying and crossing results in Morris water maze (P