Mungbean seed coat water extract inhibits inflammation in LPS-induced acute liver injury mice and LPS-stimulated RAW 246.7 macrophages via the inhibition of TAK1/IκBα/NF-κB

• Published in: Journal of food science and technology 2020-07, Vol.57 (7), p.2659-2668
• Main Authors: Sae-tan, Sudathip, Kumrungsee, Thanutchaporn, Yanaka, Noriyuki
• Format: Article
• Language: English
• Subjects: Activation; Beans; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Degradation; Flavonoids; Food Science; Gene expression; Growth factors; Inflammation; Inflammatory diseases; Injuries; Kinases; Lipopolysaccharides; Liver; Macrophages; Markers; NF-κB protein; Nitric-oxide synthase; Nutrition; Oral administration; Original; Original Article; Pathogenesis; Phosphorylation; Pretreatment; Seed coats; TAK1 protein; Transcription; Transforming growth factor-b
• ISSN: 0022-1155; 0975-8402
• DOI: 10.1007/s13197-020-04302-y

Inflammation plays an important role in pathogenesis and progression of many chronic diseases. Although, anti-inflammatory activities of mungbean have been suggested, the underlying mechanism have not been fully understood. The present study aimed to reveal the anti-inflammatory effects of mungbean seed coat water extract (MSWE) in lipopolysaccharide (LPS)-stimulated inflammation in RAW 246.7 macrophages and LPS-induced acute liver injury mice. MSWE pretreatment downregulated the elevated expression of inflammatory markers induced by LPS in the transcriptional and protein level. MSWE inhibited NF-κB activation through the suppression of phosphorylated p65 subunit, IκBα degradation, and transforming growth factor-β-activated kinases 1 (TAK1) phosphorylation in LPS-stimulated RAW 246.7 cells. Vitexin, the major flavonoid in MSWE showed similar effects. In in vivo experiments, we found that oral administration of MSWE downregulated iNOS expression in LPS-induced acute liver injury mice. The mRNA expression of inflammatory markers and macrophage infiltration was also decreased in the livers. Collectively, MSWE exerts anti-inflammatory role, in part possibly through its active compound vitexin, by inhibiting NF-κB activation via inhibition of TAK1 phosphorylation and IκBα degradation. This suggests that MSWE is beneficial to combat various inflammatory diseases.