Water is a biomarker of changes in the cellular environment in live animals

The biological processes that are associated with the physiological fitness state of a cell comprise a diverse set of molecular events. Reactive oxygen species (ROS), mitochondrial dysfunction, telomere shortening, genomic instability, epigenetic changes, protein aggregation, and down-regulation of...

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Bibliographic Details
Published in:Scientific reports 2020-06, Vol.10 (1), p.9095-9095, Article 9095
Main Authors: Siwach, Pratibha, Levy, Evgeniya, Livshits, Leonid, Feldman, Yuri, Kaganovich, Daniel
Format: Article
Language:English
Subjects:
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639/766
Aging
Aging - genetics
Aging - metabolism
Animals
Biomarkers
Biomarkers - metabolism
Body Water - metabolism
Caenorhabditis elegans - cytology
Caenorhabditis elegans - genetics
Caenorhabditis elegans - metabolism
Caenorhabditis elegans - physiology
Cytoplasm
Cytoplasm - metabolism
Dielectric Spectroscopy
Drug development
Epigenetics
Genomic Instability
Humanities and Social Sciences
Life span
Longevity - genetics
Mitochondria
multidisciplinary
Oxidative Stress - genetics
Protein Folding
Protein interaction
Proteins - metabolism
Quality control
Reactive oxygen species
Reactive Oxygen Species - metabolism
Science
Science (multidisciplinary)
Spectroscopy
Spectrum analysis
Telomere Shortening
Telomeres
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Summary:The biological processes that are associated with the physiological fitness state of a cell comprise a diverse set of molecular events. Reactive oxygen species (ROS), mitochondrial dysfunction, telomere shortening, genomic instability, epigenetic changes, protein aggregation, and down-regulation of quality control mechanisms are all hallmarks of cellular decline. Stress-related and decline-related changes can be assayed, but usually through means that are highly disruptive to living cells and tissues. Biomarkers for organismal decline and aging are urgently needed for diagnostic and drug development. Our goal in this study is to provide a proof-of-concept for a non-invasive assay of global molecular events in the cytoplasm of living animals. We show that Microwave Dielectric Spectroscopy (MDS) can be used to determine the hydration state of the intracellular environment in live C. elegans worms. MDS spectra were correlative with altered states in the cellular protein folding environment known to be associated with previously described mutations in the C. elegans lifespan and stress-response pathways.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-66022-9