The Circular RNA Landscape of Non-Small Cell Lung Cancer Cells

The class of circular RNA (circRNA) is characterized by head-to-tail bonds between exons formed by backsplicing. Here, we provide a resource of circRNA expression in a comprehensive panel of 60 lung cancer and non-transformed cell lines (FL3C dataset). RNA sequencing after depletion of ribosomal RNA...

Full description

Saved in:
Bibliographic Details
Published in:Cancers 2020-04, Vol.12 (5), p.1091
Main Authors: Van Der Steen, Nele, Lyu, Yanhong, Hitzler, Anne K, Becker, Andrea C, Seiler, Jeanette, Diederichs, Sven
Format: Article
Language:English
Subjects:
Biomarkers
Cell cycle
Cell proliferation
Circular RNA
Datasets
Exons
Genes
Genomes
Genotypes
Lung cancer
Non-small cell lung carcinoma
Peptides
RNA polymerase
rRNA
Small cell lung carcinoma
Tumor cell lines
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The class of circular RNA (circRNA) is characterized by head-to-tail bonds between exons formed by backsplicing. Here, we provide a resource of circRNA expression in a comprehensive panel of 60 lung cancer and non-transformed cell lines (FL3C dataset). RNA sequencing after depletion of ribosomal RNA quantified the expression of circRNA and linear RNA. We detected 148,811 circular RNAs quantified by 2.8 million backsplicing reads originating from 12,251 genes. The number of identified circRNAs was markedly higher using rRNA depletion compared to public polyA-enriched RNA-seq datasets. CircRNAs almost never started in the first exon nor ended in the last exon and started more frequently in earlier exons. Most circRNAs showed high cell line specificity and correlated positively with their linear RNA counterpart. Known cancer genes produced more circRNAs than non-cancer genes. Subsets of circRNAs correlated with cell proliferation, histological subtype or genotype. was translated crossing the backsplice site in two different reading frames. Overexpression of , , , , , and significantly increased colony formation. In conclusion, our data provide a comprehensive map of circRNA expression in lung cancer cells and global patterns of circRNA production as a useful resource for future research into lung cancer circRNAs.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers12051091