Doxycycline and Zinc Loaded Silica-Nanofibrous Polymers as Biomaterials for Bone Regeneration

The main target of bone tissue engineering is to design biomaterials that support bone regeneration and vascularization. Nanostructured membranes of (MMA) -co-(HEMA) /(MA) -co-(HEA) loaded with 5% wt of SiO -nanoparticles (HOOC-Si-Membrane) were doped with zinc (Zn-HOOC-Si-Membrane) or doxycycline (...

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Bibliographic Details
Published in:Polymers 2020-05, Vol.12 (5), p.1201
Main Authors: Toledano, Manuel, Toledano-Osorio, Manuel, Osorio, Raquel, Carrasco-Carmona, Álvaro, Gutiérrez-Pérez, José-Luis, Gutiérrez-Corrales, Aida, Serrera-Figallo, María-Angeles, Lynch, Christopher D, Torres-Lagares, Daniel
Format: Article
Language:English
Subjects:
Adsorption
Angiogenesis
Biological activity
Biomedical materials
Bones
Cell adhesion & migration
Computed tomography
Defects
Fluorescence
Laboratory animals
Macrophages
Mechanical properties
Membranes
Modulators
Molecular weight
Nanoparticles
Polymer blends
Polymerization
Rabbits
Regeneration (physiology)
Silicon dioxide
Tissue engineering
Toluidine blue
Topography
Zinc
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Summary:The main target of bone tissue engineering is to design biomaterials that support bone regeneration and vascularization. Nanostructured membranes of (MMA) -co-(HEMA) /(MA) -co-(HEA) loaded with 5% wt of SiO -nanoparticles (HOOC-Si-Membrane) were doped with zinc (Zn-HOOC-Si-Membrane) or doxycycline (Dox-HOOC-Si-Membrane). Critical bone defects were effectuated on six New Zealand-bred rabbit skulls and covered with the membranes. After six weeks, the bone architecture was evaluated with micro computed tomography. Three histological analyses were utilized to analyse bone regeneration, including von Kossa silver nitrate, toluidine blue and fluorescence. All membrane-treated defects exhibited higher number of osteocytes and bone perimeter than the control group without the membrane. Zn-HOOC-Si-Membranes induced higher new bone and osteoid area than those treated with HOOC-Si-Membranes, and control group, respectively. Zn-HOOC-Si-Membranes and Dox-HOOC-Si-Membranes attained the lowest ratio M1 macrophages/M2 macrophages. Dox-HOOC-Si-Membranes caused the lowest number of osteoclasts, and bone density. At the trabecular new bone, Zn-HOOC-Si-Membranes produced the highest angiogenesis, bone thickness, connectivity, junctions and branches. Zn-HOOC-Si-Membranes enhanced biological activity, attained a balanced remodeling, and achieved the greatest regenerative efficiency after osteogenesis and angiogenesis assessments. The bone-integrated Zn-HOOC-Si-Membranes can be considered as bioactive modulators provoking a M2 macrophages (pro-healing cells) increase, being a potential biomaterial for promoting bone repair.
ISSN:2073-4360
2073-4360
DOI:10.3390/POLYM12051201