Comparative Analysis of ANDE 6C Rapid DNA Analysis System and Traditional Methods

Rapid DNA analysis is an ultrafast and fully automated DNA-typing system, which can produce interpretable genetic profiles from biological samples within 90 minutes. This "swab in-profile out" method comprises DNA extraction, amplification by PCR multiplex, separation and detection of DNA...

Full description

Saved in:
Bibliographic Details
Published in:Genes 2020-05, Vol.11 (5), p.582
Main Authors: Ragazzo, Michele, Melchiorri, Stefano, Manzo, Laura, Errichiello, Valeria, Puleri, Giulio, Nicastro, Fabio, Giardina, Emiliano
Format: Article
Language:English
Subjects:
DNA - genetics
DNA - isolation & purification
DNA Fingerprinting - methods
Forensic Genetics - trends
Genotype
Humans
Microsatellite Repeats - genetics
Species Specificity
Specimen Handling
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Rapid DNA analysis is an ultrafast and fully automated DNA-typing system, which can produce interpretable genetic profiles from biological samples within 90 minutes. This "swab in-profile out" method comprises DNA extraction, amplification by PCR multiplex, separation and detection of DNA fragments by capillary electrophoresis. The aim of study was the validation of the Accelerated Nuclear DNA Equipment (ANDE) 6C system as a typing method for reference samples according to the ISO/IEC 17025 standard. Here, we report the evaluation of the validity and reproducibility of results by the comparison of the genetic profiles generated by the ANDE 6C System with those generated by standard technologies. A quantity of 104 buccal swabs were analyzed both through the ANDE 6C technology and the traditional method (DNA extraction and quantification, amplification and separation by capillary electrophoresis). Positive typing was observed in 97% of cases for ANDE 6C technology with only three buccal swabs failing to reveal interpretable signals. Concordance was determined by comparing the allele calls generated by ANDE 6C and conventional technology. Comparison of 2800 genotypes revealed a concordance rate of 99.96%. These results met the ISO/IEC 17025 requirements, enabling us to receive the accreditation for this method. Finally, rapid technology has certainly reached a level of reliability which has made its use in laboratories of forensic genetics a reality.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes11050582