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A Protein Epitope Targeted by the Antibody Response to Kawasaki Disease

Abstract Background Kawasaki disease (KD) is the leading cause of childhood acquired heart disease in developed nations and can result in coronary artery aneurysms and death. Clinical and epidemiologic features implicate an infectious cause but specific antigenic targets of the disease are unknown....

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Bibliographic Details
Published in:The Journal of infectious diseases 2020-06, Vol.222 (1), p.158-168
Main Authors: Rowley, Anne H, Baker, Susan C, Arrollo, David, Gruen, Leah J, Bodnar, Tetyana, Innocentini, Nancy, Hackbart, Matthew, Cruz-Pulido, Yazmin E, Wylie, Kristine M, Kim, Kwang-Youn A, Shulman, Stanford T
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Language:English
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Summary:Abstract Background Kawasaki disease (KD) is the leading cause of childhood acquired heart disease in developed nations and can result in coronary artery aneurysms and death. Clinical and epidemiologic features implicate an infectious cause but specific antigenic targets of the disease are unknown. Peripheral blood plasmablasts are normally highly clonally diverse but the antibodies they encode are approximately 70% antigen-specific 1–2 weeks after infection. Methods We isolated single peripheral blood plasmablasts from children with KD 1–3 weeks after onset and prepared 60 monoclonal antibodies (mAbs). We used the mAbs to identify their target antigens and assessed serologic response among KD patients and controls to specific antigen. Results Thirty-two mAbs from 9 of 11 patients recognize antigen within intracytoplasmic inclusion bodies in ciliated bronchial epithelial cells of fatal cases. Five of these mAbs, from 3 patients with coronary aneurysms, recognize a specific peptide, which blocks binding to inclusion bodies. Sera from 5/8 KD patients day ≥ 8 after illness onset, compared with 0/17 infant controls (P 
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/jiaa066