Restriction site-associated DNA sequencing (RAD-seq) analysis in Pacific oyster Crassostrea gigas based on observation of individual sex changes

The diverse modes of sexual reproduction in Bivalvia make it an excellent clade to understand the evolution of sex and sex determination. The cosmopolitan Pacific oyster Crassostrea gigas is an ideal model for bivalve sex determination studies because of its complicated sexuality, including dioecy,...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2020-06, Vol.10 (1), p.9873-9873, Article 9873
Main Authors: Yue, Chenyang, Li, Qi, Yu, Hong, Liu, Shikai, Kong, Lingfeng
Format: Article
Language:English
Subjects:
631/208/721
631/208/8
Animals
Bivalvia
Chromosomes
Crassostrea - genetics
Crassostrea gigas
Deoxyribonucleic acid
DNA
DNA sequencing
Female
Hermaphroditism
Heterozygosity
Humanities and Social Sciences
Male
Models, Biological
Mollusks
multidisciplinary
Oysters
Physical growth
Polymorphism, Single Nucleotide
Protandry
RNA - chemistry
RNA - metabolism
RNA-Seq
Science
Science (multidisciplinary)
Sex chromosomes
Sex determination
Sex Determination Processes - genetics
Sex linkage
Sex reversal
Sexual reproduction
Sexuality
Shellfish Proteins - metabolism
Single-nucleotide polymorphism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The diverse modes of sexual reproduction in Bivalvia make it an excellent clade to understand the evolution of sex and sex determination. The cosmopolitan Pacific oyster Crassostrea gigas is an ideal model for bivalve sex determination studies because of its complicated sexuality, including dioecy, sex change and rare hermaphroditism. A major barrier to C. gigas sex determination study has been the lack of information on the type of sex determination. To identify its sex-determining system, sex observation by following the same individual in two consecutive years was conducted on 760 oysters from distinct populations. Stable sexuality and sex reversal in both directions were observed, which provides a case against the protandry of C. gigas . Restriction site-associated DNA sequencing (RAD-seq) based on 26 samples with unchanged and converted sexualities was carried out for identifying sex-linked marker. One SNP Cgsl-40 was proved to be sex-related, but sex-biased heterozygosity varied between populations for RAD-seq and validation, showing no evidence for sex chromosomes or single-locus models for C. gigas primary sex determination. Information obtained in our study provides novel insight into sex determination mechanism in C. gigas .
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-67007-4