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Cell Fate Potential of NG2 Progenitors

Determining the origin of different glial subtypes is crucial to understand glial heterogeneity, and to enhance our knowledge of glial and progenitor cell behavior in embryos and adults. NG2-glia are homogenously distributed in a grid-like manner in both, gray and white matter of the adult brain. Wh...

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Published in:Scientific reports 2020-06, Vol.10 (1), p.9876-9876, Article 9876
Main Authors: Sánchez-González, Rebeca, Bribián, Ana, López-Mascaraque, Laura
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description Determining the origin of different glial subtypes is crucial to understand glial heterogeneity, and to enhance our knowledge of glial and progenitor cell behavior in embryos and adults. NG2-glia are homogenously distributed in a grid-like manner in both, gray and white matter of the adult brain. While some NG2-glia in the CNS are responsible for the generation of mature oligodendrocytes (OPCs), most of them do not differentiate and they can proliferate outside of adult neurogenic niches. Thus, NG2-glia constitute a heterogeneous population containing different subpopulations with distinct functions. We hypothesized that their diversity emerges from specific progenitors during development, as occurs with other glial cell subtypes. To specifically target NG2-pallial progenitors and to define the NG2-glia lineage, as well as the NG2-progenitor potential, we designed two new StarTrack strategies using the NG2 promoter. These approaches label NG2 expressing progenitor cells, permitting the cell fates of these NG2 progenitors to be tracked in vivo . StarTrack labelled cells producing different neural phenotypes in different regions depending on the age targeted, and the strategy selected. This specific genetic targeting of neural progenitors in vivo has provided new data on the heterogeneous pool of NG2 progenitors at both embryonic and postnatal ages.
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subjects 631/378/2571
631/378/2571/2578
631/378/2571/2579
631/378/2571/2580
631/378/2582
Animals
Antigens - genetics
Antigens - metabolism
Brain - metabolism
Cell Differentiation
Cell fate
Embryo, Mammalian - metabolism
Embryos
Genomes
Glial cells
Heterogeneity
Humanities and Social Sciences
Localization
Mice
Mice, Inbred C57BL
Morphology
multidisciplinary
Neural stem cells
Neuroglia - cytology
Neuroglia - metabolism
Neuronal-glial interactions
Oligodendrocytes
Oligodendroglia - cytology
Oligodendroglia - metabolism
Phenotypes
Plasmids
Plasmids - genetics
Plasmids - metabolism
Population
Progenitor cells
Promoter Regions, Genetic
Proteoglycans - genetics
Proteoglycans - metabolism
Science
Science (multidisciplinary)
Stem Cells - cytology
Stem Cells - metabolism
Subpopulations
Substantia alba
title Cell Fate Potential of NG2 Progenitors
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