hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue

Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to impl...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2020-06, Vol.10 (1), p.9950-9950, Article 9950
Main Authors: Hildebrand, Sven, Schueth, Anna, Wangenheim, Klaus von, Mattheyer, Christian, Pampaloni, Francesco, Bratzke, Hansjürgen, Roebroeck, Alard F., Galuske, Ralf A. W.
Format: Article
Language:English
Subjects:
14/34
14/63
631/378/3920
692/698/1688/64
Affinity Labels - chemistry
Animals
Brain
Brain - anatomy & histology
Brain - cytology
Carbocyanines - chemistry
Fluorescent Dyes - chemistry
Formaldehyde - chemistry
Humanities and Social Sciences
Humans
Lipids
Lipids - chemistry
Lipophilic
Lysine
Lysine - chemistry
Mice
multidisciplinary
Perchlorate
Perchloric acid
Periodic Acid - chemistry
Science
Science (multidisciplinary)
Swine
Tissue Fixation - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-66999-3