Proliferation, Characterization and Differentiation Potency of Adipose Tissue-Derived Mesenchymal Stem Cells (AT-MSCs) Cultured in Fresh Frozen and non-Fresh Frozen Plasma

Mesenchymal stem cells (MSCs) have unique properties, including high proliferation rates, self-renewal, and multilineage differentiation ability. Their characteristics are affected by increasing age and microenvironment. This research is aimed to determine the proliferation, characteristics and diff...

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Published in:International journal of molecular and cellular medicine 2019, Vol.8 (4), p.283-294
Main Authors: Widowati Wahyu, Rachmawati, Noverina, Wireni, Ayuningtyas, Kurniawan Dedy, Kusuma Hanna Sari Widya, Seila, Arumwardana, Artie Dwi Surya, Sholihah Ika Adhani, Handayani Rr. Anisa Siwianti, Laksmitawati Dian Ratih, Ratih, Rinendyaputri, Rilianawati, Rilianawati, Ahmad, Faried
Format: Article
Language:English
Subjects:
Adipocytes
Antibiotics
Body fat
Cell growth
Morphology
Original
Plasma
Standard deviation
Statistical analysis
Stem cells
Variance analysis
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Summary:Mesenchymal stem cells (MSCs) have unique properties, including high proliferation rates, self-renewal, and multilineage differentiation ability. Their characteristics are affected by increasing age and microenvironment. This research is aimed to determine the proliferation, characteristics and differentiation capacity of adipose tissue-derived (AT)-MSCs at many passages with different media. The cell proliferation capacity was assayed using trypan blue. MSCs characterization (CD90, CD44, CD105, CD73, CD11b, CD19, CD34, CD45, and HLA-DR) was performed by flow cytometry, and cell differentiation was determined by specific stainings. Population doubling time (PDT) of AT-MSCs treated with fresh frozen plasma (FFP) and non-FFP increased in the late passage (P) (P15 FFP was 22.67 ± 7.01 days and non-FFP was 19.65 ± 2.27 days). Cumulative cell number was significantly different between FFP and non-FFP at P5, 10, 15. AT-MSCs at P4-15 were positive for CD90, CD44, CD105, and CD73, and negative for CD11b, CD19, CD34, CD45, and HLA-DR surface markers. AT-MSCs at P5, 10, 15 had potential toward adipogenic, chondrogenic, and osteogenic differentiation. Therefore, PDT was affected by increased age but no difference was observed in morphology, surface markers and differentiation capacity among passages. Cumulative cell number in FFP was higher in comparison with non-FFP in P5, 10, 15. Our data suggest that FFP may replace FBS for culturing MSCs.
ISSN:2251-9637
2251-9645
DOI:10.22088/IJMCM.BUMS.8.4.283