Co-opted Cellular Sac1 Lipid Phosphatase and PI(4)P Phosphoinositide Are Key Host Factors during the Biogenesis of the Tombusvirus Replication Compartment

• Published in: Journal of virology 2020-06, Vol.94 (12)
• Main Authors: Sasvari, Zsuzsanna, Lin, Wenwu, Inaba, Jun-Ichi, Xu, Kai, Kovalev, Nikolay, Nagy, Peter D
• Format: Article
• Language: English
• Subjects: 1-Phosphatidylinositol 4-Kinase - genetics; 1-Phosphatidylinositol 4-Kinase - metabolism; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis - virology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Endosomes - metabolism; Gene Expression Regulation; Host-Pathogen Interactions - genetics; Nicotiana - genetics; Nicotiana - metabolism; Nicotiana - virology; Phosphatidylethanolamines - genetics; Phosphatidylethanolamines - metabolism; Phosphatidylinositol Phosphates - metabolism; Phosphoric Monoester Hydrolases - deficiency; Phosphoric Monoester Hydrolases - genetics; Phosphoric Monoester Hydrolases - metabolism; Plant Cells - metabolism; Plant Cells - virology; Plant Leaves - genetics; Plant Leaves - metabolism; Plant Leaves - virology; Protein Isoforms - genetics; Protein Isoforms - metabolism; Protoplasts - metabolism; Qa-SNARE Proteins - genetics; Qa-SNARE Proteins - metabolism; rab5 GTP-Binding Proteins - genetics; rab5 GTP-Binding Proteins - metabolism; RNA-Dependent RNA Polymerase - genetics; RNA-Dependent RNA Polymerase - metabolism; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae - virology; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Signal Transduction; Spotlight; Sterols - metabolism; Tombusvirus - genetics; Tombusvirus - metabolism; Viral Proteins - genetics; Viral Proteins - metabolism; Virus Replication - genetics; Virus-Cell Interactions
• ISSN: 0022-538X; 1098-5514
• DOI: 10.1128/jvi.01979-19

Positive-strand RNA [(+)RNA] viruses assemble numerous membrane-bound viral replicase complexes (VRCs) with the help of viral replication proteins and co-opted host proteins within large viral replication compartments in the cytosol of infected cells. In this study, we found that deletion or depletion of Sac1 phosphatidylinositol 4-phosphate [PI(4)P] phosphatase reduced tomato bushy stunt virus (TBSV) replication in yeast ( ) and plants. We demonstrate a critical role for Sac1 in TBSV replicase assembly in a cell-free replicase reconstitution assay. The effect of Sac1 seems to be direct, based on its interaction with the TBSV p33 replication protein, its copurification with the tombusvirus replicase, and its presence in the virus-induced membrane contact sites and within the TBSV replication compartment. The proviral functions of Sac1 include manipulation of lipid composition, sterol enrichment within the VRCs, and recruitment of additional host factors into VRCs. Depletion of Sac1 inhibited the recruitment of Rab5 GTPase-positive endosomes and enrichment of phosphatidylethanolamine in the viral replication compartment. We propose that Sac1 might be a component of the assembly hub for VRCs, likely in collaboration with the co-opted the syntaxin18-like Ufe1 SNARE protein within the TBSV replication compartments. This work also led to demonstration of the enrichment of PI(4)P phosphoinositide within the replication compartment. Reduction in the PI(4)P level due to chemical inhibition in plant protoplasts; depletion of two PI(4)P kinases, Stt4p and Pik1p; or sequestration of free PI(4)P via expression of a PI(4)P-binding protein in yeast strongly inhibited TBSV replication. Altogether, Sac1 and PI(4)P play important proviral roles during TBSV replication. Replication of positive-strand RNA viruses depends on recruitment of host components into viral replication compartments or organelles. Using TBSV, we uncovered the critical roles of Sac1 PI(4)P phosphatase and its substrate, PI(4)P phosphoinositide, in promoting viral replication. Both Sac1 and PI(4)P are recruited to the site of viral replication to facilitate the assembly of the viral replicase complexes, which perform viral RNA replication. We found that Sac1 affects the recruitment of other host factors and enrichment of phosphatidylethanolamine and sterol lipids within the subverted host membranes to promote optimal viral replication. In summary, this work demonstrates the novel functions of Sac1 and P