Features of p53 protein distribution in the corneal epithelium and corneal tear film

• Published in: Scientific reports 2020-06, Vol.10 (1), p.10051, Article 10051
• Main Authors: Tendler, Yevgeny, Panshin, Alexander
• Format: Article
• Language: English
• Subjects: 14/1; 14/28; 631/67; 631/80; 82/51; 82/79; Animals; Cell proliferation; Cornea; Cytoplasm; Epithelium; Epithelium, Corneal - metabolism; Exosomes; Exosomes - metabolism; Eye lens; Humanities and Social Sciences; Immunohistochemistry; Iris; Iris - metabolism; Lens, Crystalline - metabolism; MDM2 protein; Mice; Microscopy, Electron; Mucin; multidisciplinary; p53 Protein; Proteins; Proto-Oncogene Proteins c-mdm2 - metabolism; Rats; Retina; Retina - metabolism; Science; Science (multidisciplinary); Tears - metabolism; Tissue Distribution; Tumor suppressor genes; Tumor Suppressor Protein p53 - metabolism; Up-Regulation
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-67206-z

Tumor suppressor protein p53 is the key factor in the regulation of cell proliferation. Its concentration is low in the cytoplasm of most cell types. However, in corneal epithelium cells, abnormally high p53 content is detected. The aim of the present study was to characterize p53 distribution in the corneal epithelium. For this purpose, immunohistochemistry, western blot analysis and electronic microscope examinations were performed. A low level of p53 was identified in the lens, iris and retina; by contrast, a significantly high concentration of this protein was observed in the corneal epithelium. In opposite, MDM2 was identified in the lens, iris and retina while it is completely absent in the corneal epithelium. In addition, we found a significant amount of exosomes and other microvesicles containing p53 in the corneal mucin layer. We thus hypothesize that a significantly high level of p53 was caused by a combination of absents of MDM2 in parallel with p53 microvesicles storage.