Inhibitory Effect of Ursolic Acid on Ultraviolet B Radiation-Induced Oxidative Stress and Proinflammatory Response-Mediated Senescence in Human Skin Dermal Fibroblasts

• Published in: Oxidative medicine and cellular longevity 2020, Vol.2020 (2020), p.1-17
• Main Authors: Akhtar, Saeed, Masmali, Ali M., Khan, Adnan A., Subramanian, Umadevi, Prasad, Nagarajan Rajendra, Samivel, Ramachandran, Almubrad, Turki
• Format: Article
• Language: English
• Subjects: Analysis; Antioxidants; Apoptosis; bcl-2-Associated X Protein - metabolism; Caspase 3 - metabolism; Cell culture; Cell Nucleus - drug effects; Cell Nucleus - metabolism; Cell Nucleus - radiation effects; Cell Survival - drug effects; Cellular Senescence - drug effects; Cellular Senescence - radiation effects; Collagen; Deoxyribonucleic acid; Dermatologic agents; Dermatology; Dermis - pathology; DNA; DNA Damage; Ethylenediaminetetraacetic acid; Extracellular matrix; Fibroblasts; Fibroblasts - drug effects; Fibroblasts - metabolism; Fibroblasts - pathology; Formulae, receipts, prescriptions; Free radicals; Growth factors; Humans; Inflammation; Inflammation - pathology; Lipid peroxidation; Matrix Metalloproteinase 2 - genetics; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 9 - genetics; Matrix Metalloproteinase 9 - metabolism; Membrane Potential, Mitochondrial - drug effects; NF-kappa B - metabolism; Oxidative stress; Oxidative Stress - drug effects; Oxidative Stress - radiation effects; Phytochemicals; Prevention; Radiation; Reactive oxygen species; Reactive Oxygen Species - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Senescence; Signal transduction; Skin; Thiobarbituric Acid Reactive Substances - metabolism; Triterpenes - chemistry; Triterpenes - pharmacology; Tumor Necrosis Factor-alpha - metabolism; Tumor Suppressor Protein p53 - metabolism; Ultraviolet radiation; Ultraviolet Rays; Ursolic Acid
• ISSN: 1942-0900; 1942-0994
• DOI: 10.1155/2020/1246510

Ultraviolet radiation is an environmental carcinogenic agent that enhances inflammation and immunological reactions in the exposed human skin cells leading to oxidative photoaging of the epidermal and dermal segment. In the present study, we investigated the protective role of ursolic acid (UA) against ultraviolet B (UVB) radiation- induced photoaging an in vitro model of human skin dermal fibroblasts. UA-pretreated human skin dermal fibroblast (HDF) cells were exposed to UVB radiation to evaluated cell viability, reactive oxygen species (ROS), mitochondrial membrane potential, lipid peroxidation, antioxidant status, DNA damage, proinflammatory response, apoptotic induction, and matrix metalloproteinase (MMP) alteration. The UA pretreatment of HDFs mitigated the UVB irradiation-induced cytotoxicity, ROS generation, and mitochondrial membrane potential alteration and lipid peroxidation, depletion of antioxidant status, DNA damage, and apoptotic induction. UA pretreatment of HDFs also attenuated the UVB-induced expression of inflammatory (TNF-α and NF-κB) and apoptotic (p53, Bax, and caspase-3) and MMPs (MMP-2 and MMP-9) and enhanced the Bcl-2 protein levels in 20 μM UA treatment, when compared to concentrations. Hence, these results revealed that UA has the potential to mitigate UVB-induced extracellular damage by interfering with the ROS-mediated apoptotic induction and photoaging senescence and thus is a potential therapeutic agent to protect the skin against UVB-irradiation induced photooxidative damage.