Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification

This study investigated a method using loop-mediated isothermal amplification (LAMP) for the rapid detection of cnm-positive Streptococcus mutans (S. mutans) associated with cerebral microhemorrhage. LAMP amplified the cnm gene plasmid vector, but not human or microbial genomic DNA. The cnm DNA of t...

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Bibliographic Details
Published in:Analytical biochemistry 2020-09, Vol.605, p.113812-113812, Article 113812
Main Authors: Kitagawa, Masae, Nagamine, Kentaro, Oka, Hiroko, Ouhara, Kazuhisa, Ogawa, Ikuko, Komatsuzawa, Hitoshi, Kurihara, Hidemi
Format: Article
Language:English
Subjects:
Adhesins, Bacterial - analysis
Carrier Proteins - analysis
cnm gene
Humans
Loop-mediated isothermal amplification
Molecular Diagnostic Techniques
Nucleic Acid Amplification Techniques
Saliva - microbiology
Streptococcus mutans
Streptococcus mutans - isolation & purification
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Summary:This study investigated a method using loop-mediated isothermal amplification (LAMP) for the rapid detection of cnm-positive Streptococcus mutans (S. mutans) associated with cerebral microhemorrhage. LAMP amplified the cnm gene plasmid vector, but not human or microbial genomic DNA. The cnm DNA of the cnm-positive S. mutans strain was detected in saliva without DNA extraction after 1 day of culture. This method resulted in a cnm-positive rate of 26.4% in 102 samples, which was higher than that obtained with conventional PCR. In conclusion, LAMP may be used for the detection of cnm-positive S. mutans in a large number of samples. •Easy detection of cnm gene related to cerebral hemorrhage.•Non-invasive sampling using saliva.•Reduced detection time due to shorter culture period and not extracting DNA.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2020.113812