Histopathologic, immunophenotypic, and proteomics characteristics of low-grade phyllodes tumor and fibroadenoma: more similarities than differences

Distinguishing low-grade phyllodes tumor from fibroadenoma is practically challenging due to their overlapping histologic features. However, the final interpretation is essential to surgeons, who base their management on the final pathology report. Patients who receive a diagnosis of fibroadenoma mi...

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Bibliographic Details
Published in:NPJ breast cancer 2020-06, Vol.6 (1), p.27-27, Article 27
Main Authors: Zhang, Lingxin, Yang, Chen, Pfeifer, John D., Caprioli, Richard M., Judd, Audra M., Patterson, Nathan H., Reyzer, Michelle L., Norris, Jeremy L., Maluf, Horacio M.
Format: Article
Language:English
Subjects:
631/67/1347
692/308/409
692/4028/546
Age
Biomedical and Life Sciences
Biomedicine
Biopsy
Breast cancer
Cancer Research
Cell Biology
Feasibility studies
Histology
Human Genetics
Mass spectrometry
Medical research
Mortality
Oncology
Pathology
Patients
Proteomics
Scientific imaging
Tumors
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Summary:Distinguishing low-grade phyllodes tumor from fibroadenoma is practically challenging due to their overlapping histologic features. However, the final interpretation is essential to surgeons, who base their management on the final pathology report. Patients who receive a diagnosis of fibroadenoma might not undergo any additional intervention while lumpectomy with wide margins is the standard of care for phyllodes tumor, which can have significant cosmetic consequences. We studied the clinical, immunophenotypic, and proteomics profiles of 31 histologically confirmed low-grade phyllodes tumor and 30 fibroadenomas. Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) and immunohistochemistry for Ki-67, p53, β-catenin, and E-cadherin were performed on all cases. After the mass spectra for all 31 cases of low-grade phyllodes tumor and 30 cases of fibroadenoma were collected, an average peak value for all cases was generated. There was no significant difference in the overall mass spectra pattern in any of the peaks identified. There was also overlap in the percentage of cells staining positive for Ki-67, p53, β-catenin, and E-cadherin. The two groups of patients showed no statistically significant difference in age, tumor size, or disease-free survival. Neither group developed malignant transformation, distant metastases, or disease-related mortality. We have demonstrated low-grade phyllodes tumor and fibroadenoma to show significant overlapping clinical and proteomics features.
ISSN:2374-4677
2374-4677
DOI:10.1038/s41523-020-0169-8