Application of Sysmex XN-Series Automated Haematology Analyser in the Rapid Detection of Malaria

Malaria, an important parasitic disease worldwide, still has diagnostic challenges in the laboratory. Many studies have been conducted on the detection ability of haematology analysers for malaria. We evaluated the Sysmex XN-series analyser as a tool for detection of malaria by analysing the leukocy...

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Published in:Indian journal of hematology & blood transfusion 2020-07, Vol.36 (3), p.512-518
Main Authors: Ningombam, Aparna, Sarkar, Abhirup, Acharya, Shreyam, Chopra, Anita, Kumar, Kundan, Subramanian, Arulselvi
Format: Article
Language:English
Subjects:
Blood Transfusion Medicine
Diagnostic tests
Hematology
Human Genetics
Malaria
Medical diagnosis
Medicine
Medicine & Public Health
Oncology
Original
Original Article
Parasites
Parasitic diseases
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Summary:Malaria, an important parasitic disease worldwide, still has diagnostic challenges in the laboratory. Many studies have been conducted on the detection ability of haematology analysers for malaria. We evaluated the Sysmex XN-series analyser as a tool for detection of malaria by analysing the leukocyte cell population data (LCPD), scattergrams and associated Flow Cytometry Standard (FCS) data from both the WNR (white cell nucleated) and WDF (white cell differential) channels. 1281 clinically suspected cases of malaria were screened for malaria by peripheral blood smear examination and were run in the Sysmex XN-1000 for analysis of haematological parameter data, LCPD, all the scattergrams and FCS data. 1281 clinically suspected cases of malaria were screened for malaria by peripheral blood smear examination and were run in the Sysmex XN-1000 for analysis of haematological parameter data, LCPD, all the scattergrams and FCS data. 48 cases had malarial parasite on microscopy; of which, 41 cases were of Plasmodium vivax , 6 cases of Plasmodium falciparum and 1 case of mixed infection. 46 malaria-positive samples showed certain patterns of clusters in the scattergrams of both WDF and WNR channels. A case with only a few ring forms of P. vivax and another with very low parasite load having only gametocyte of P. falciparum didn’t show the distinctive cluster. The most distinctive clusters for all other cases were seen in WNR (SFL-SSC) and WNR (SSC-FSC) scattergrams. FCS data for the same were analysed to gate for those events. The gated events correlated (Spearman ρ = 0.77, p  
ISSN:0971-4502
0974-0449
0974-0449
0971-4502
DOI:10.1007/s12288-020-01276-x