Loading…
SARS-CoV-2 does not replicate in embryonated hen's eggs or in MDCK cell lines
The advent of COVID-19, has posed a risk that human respiratory samples containing human influenza viruses may also contain SARS-CoV-2. This potential risk may lead to SARS-CoV-2 contaminating conventional influenza vaccine production platforms as respiratory samples are used to directly inoculate e...
Saved in:
| Published in: | Euro surveillance : bulletin européen sur les maladies transmissibles 2020-06, Vol.25 (25), p.10 |
|---|---|
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c469t-c217c1cc44283ad08c4289050cec2dff7c2332a158e0c201a4f37bf63534008d3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c469t-c217c1cc44283ad08c4289050cec2dff7c2332a158e0c201a4f37bf63534008d3 |
| container_end_page | |
| container_issue | 25 |
| container_start_page | 10 |
| container_title | Euro surveillance : bulletin européen sur les maladies transmissibles |
| container_volume | 25 |
| creator | Barr, Ian G Rynehart, Cleve Whitney, Paul Druce, Julian |
| description | The advent of COVID-19, has posed a risk that human respiratory samples containing human influenza viruses may also contain SARS-CoV-2. This potential risk may lead to SARS-CoV-2 contaminating conventional influenza vaccine production platforms as respiratory samples are used to directly inoculate embryonated hen's eggs and continuous cell lines that are used to isolate and produce influenza vaccines. We investigated the ability of these substrates to propagate SARS-CoV-2 and found that neither could support SARS-CoV-2 replication. |
| doi_str_mv | 10.2807/1560-7917.ES.2020.25.25.2001122 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7331139</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2418147768</sourcerecordid><originalsourceid>FETCH-LOGICAL-c469t-c217c1cc44283ad08c4289050cec2dff7c2332a158e0c201a4f37bf63534008d3</originalsourceid><addsrcrecordid>eNpdkV9LwzAUxYMoOqdfQQI-zJfOm6Rt2hdF6vyDDsGqryFL01npkpm0gt_e1s2hwoXc5J57yOGH0IjAmCbAT0kUQ8BTwseTfEyBds_RdwEQQukWGmwU27_6PbTv_RtAyCClu2iP0ZiwlPEBmuYXj3mQ2ZeA4sJqj41tsNPLulKy0bgyWC9m7tOa7lbgV21GHuv53GPr-uH0MrvDStc1riuj_QHaKWXt9eH6HKLnq8lTdhPcP1zfZhf3gQrjtAkUJVwRpcKQJkwWkKiuSSECpRUtypIryhiVJEo0KApEhiXjszJmEQsBkoIN0dnKd9nOFrpQ2jRO1mLpqoV0n8LKSvydmOpVzO2H4IyRPvkQnawNnH1vtW_EovJ9Dmm0bb2gIUk5iRmBTnr8T_pmW2e6eL0qISHncdKpzlcq5az3TpebzxAQPTrRwxA9DDHJRY9O0Oi7Vug6h6PfmTb7P6zYFxylk0A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2418147768</pqid></control><display><type>article</type><title>SARS-CoV-2 does not replicate in embryonated hen's eggs or in MDCK cell lines</title><source>PubMed Central</source><creator>Barr, Ian G ; Rynehart, Cleve ; Whitney, Paul ; Druce, Julian</creator><creatorcontrib>Barr, Ian G ; Rynehart, Cleve ; Whitney, Paul ; Druce, Julian</creatorcontrib><description>The advent of COVID-19, has posed a risk that human respiratory samples containing human influenza viruses may also contain SARS-CoV-2. This potential risk may lead to SARS-CoV-2 contaminating conventional influenza vaccine production platforms as respiratory samples are used to directly inoculate embryonated hen's eggs and continuous cell lines that are used to isolate and produce influenza vaccines. We investigated the ability of these substrates to propagate SARS-CoV-2 and found that neither could support SARS-CoV-2 replication.</description><identifier>ISSN: 1560-7917</identifier><identifier>ISSN: 1025-496X</identifier><identifier>EISSN: 1560-7917</identifier><identifier>DOI: 10.2807/1560-7917.ES.2020.25.25.2001122</identifier><identifier>PMID: 32613937</identifier><language>eng</language><publisher>Sweden: Centre Europeen pour la Surveillance Epidemiologique du SIDA (European Centre for the Epidemiological Monitoring of AIDS)</publisher><subject>Animals ; Betacoronavirus ; Cell Line ; Chickens - immunology ; Chickens - virology ; Coronavirus - physiology ; Coronavirus Infections - epidemiology ; Coronavirus Infections - virology ; COVID-19 ; Dogs ; Eggs ; Humans ; Immunization ; Influenza Vaccines - immunology ; Influenza, Human - prevention & control ; Madin Darby Canine Kidney Cells ; Pandemics ; Pneumonia, Viral ; Rapid Communication ; Receptors, Virus - metabolism ; SARS-CoV-2 ; Severe Acute Respiratory Syndrome ; Severe acute respiratory syndrome coronavirus 2 ; Studies ; Vaccines ; Virology ; Virus Cultivation - methods ; Virus Replication ; Zoonoses</subject><ispartof>Euro surveillance : bulletin européen sur les maladies transmissibles, 2020-06, Vol.25 (25), p.10</ispartof><rights>Copyright Centre Europeen pour la Surveillance Epidemiologique du SIDA (European Centre for the Epidemiological Monitoring of AIDS) Jun 25, 2020</rights><rights>This article is copyright of the authors or their affiliated institutions, 2020. 2020 The authors or their affiliated institutions</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-c217c1cc44283ad08c4289050cec2dff7c2332a158e0c201a4f37bf63534008d3</citedby><cites>FETCH-LOGICAL-c469t-c217c1cc44283ad08c4289050cec2dff7c2332a158e0c201a4f37bf63534008d3</cites><orcidid>0000-0002-7351-418X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331139/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331139/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32613937$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barr, Ian G</creatorcontrib><creatorcontrib>Rynehart, Cleve</creatorcontrib><creatorcontrib>Whitney, Paul</creatorcontrib><creatorcontrib>Druce, Julian</creatorcontrib><title>SARS-CoV-2 does not replicate in embryonated hen's eggs or in MDCK cell lines</title><title>Euro surveillance : bulletin européen sur les maladies transmissibles</title><addtitle>Euro Surveill</addtitle><description>The advent of COVID-19, has posed a risk that human respiratory samples containing human influenza viruses may also contain SARS-CoV-2. This potential risk may lead to SARS-CoV-2 contaminating conventional influenza vaccine production platforms as respiratory samples are used to directly inoculate embryonated hen's eggs and continuous cell lines that are used to isolate and produce influenza vaccines. We investigated the ability of these substrates to propagate SARS-CoV-2 and found that neither could support SARS-CoV-2 replication.</description><subject>Animals</subject><subject>Betacoronavirus</subject><subject>Cell Line</subject><subject>Chickens - immunology</subject><subject>Chickens - virology</subject><subject>Coronavirus - physiology</subject><subject>Coronavirus Infections - epidemiology</subject><subject>Coronavirus Infections - virology</subject><subject>COVID-19</subject><subject>Dogs</subject><subject>Eggs</subject><subject>Humans</subject><subject>Immunization</subject><subject>Influenza Vaccines - immunology</subject><subject>Influenza, Human - prevention & control</subject><subject>Madin Darby Canine Kidney Cells</subject><subject>Pandemics</subject><subject>Pneumonia, Viral</subject><subject>Rapid Communication</subject><subject>Receptors, Virus - metabolism</subject><subject>SARS-CoV-2</subject><subject>Severe Acute Respiratory Syndrome</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Studies</subject><subject>Vaccines</subject><subject>Virology</subject><subject>Virus Cultivation - methods</subject><subject>Virus Replication</subject><subject>Zoonoses</subject><issn>1560-7917</issn><issn>1025-496X</issn><issn>1560-7917</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpdkV9LwzAUxYMoOqdfQQI-zJfOm6Rt2hdF6vyDDsGqryFL01npkpm0gt_e1s2hwoXc5J57yOGH0IjAmCbAT0kUQ8BTwseTfEyBds_RdwEQQukWGmwU27_6PbTv_RtAyCClu2iP0ZiwlPEBmuYXj3mQ2ZeA4sJqj41tsNPLulKy0bgyWC9m7tOa7lbgV21GHuv53GPr-uH0MrvDStc1riuj_QHaKWXt9eH6HKLnq8lTdhPcP1zfZhf3gQrjtAkUJVwRpcKQJkwWkKiuSSECpRUtypIryhiVJEo0KApEhiXjszJmEQsBkoIN0dnKd9nOFrpQ2jRO1mLpqoV0n8LKSvydmOpVzO2H4IyRPvkQnawNnH1vtW_EovJ9Dmm0bb2gIUk5iRmBTnr8T_pmW2e6eL0qISHncdKpzlcq5az3TpebzxAQPTrRwxA9DDHJRY9O0Oi7Vug6h6PfmTb7P6zYFxylk0A</recordid><startdate>20200625</startdate><enddate>20200625</enddate><creator>Barr, Ian G</creator><creator>Rynehart, Cleve</creator><creator>Whitney, Paul</creator><creator>Druce, Julian</creator><general>Centre Europeen pour la Surveillance Epidemiologique du SIDA (European Centre for the Epidemiological Monitoring of AIDS)</general><general>European Centre for Disease Prevention and Control (ECDC)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7351-418X</orcidid></search><sort><creationdate>20200625</creationdate><title>SARS-CoV-2 does not replicate in embryonated hen's eggs or in MDCK cell lines</title><author>Barr, Ian G ; Rynehart, Cleve ; Whitney, Paul ; Druce, Julian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-c217c1cc44283ad08c4289050cec2dff7c2332a158e0c201a4f37bf63534008d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Betacoronavirus</topic><topic>Cell Line</topic><topic>Chickens - immunology</topic><topic>Chickens - virology</topic><topic>Coronavirus - physiology</topic><topic>Coronavirus Infections - epidemiology</topic><topic>Coronavirus Infections - virology</topic><topic>COVID-19</topic><topic>Dogs</topic><topic>Eggs</topic><topic>Humans</topic><topic>Immunization</topic><topic>Influenza Vaccines - immunology</topic><topic>Influenza, Human - prevention & control</topic><topic>Madin Darby Canine Kidney Cells</topic><topic>Pandemics</topic><topic>Pneumonia, Viral</topic><topic>Rapid Communication</topic><topic>Receptors, Virus - metabolism</topic><topic>SARS-CoV-2</topic><topic>Severe Acute Respiratory Syndrome</topic><topic>Severe acute respiratory syndrome coronavirus 2</topic><topic>Studies</topic><topic>Vaccines</topic><topic>Virology</topic><topic>Virus Cultivation - methods</topic><topic>Virus Replication</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barr, Ian G</creatorcontrib><creatorcontrib>Rynehart, Cleve</creatorcontrib><creatorcontrib>Whitney, Paul</creatorcontrib><creatorcontrib>Druce, Julian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Euro surveillance : bulletin européen sur les maladies transmissibles</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barr, Ian G</au><au>Rynehart, Cleve</au><au>Whitney, Paul</au><au>Druce, Julian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SARS-CoV-2 does not replicate in embryonated hen's eggs or in MDCK cell lines</atitle><jtitle>Euro surveillance : bulletin européen sur les maladies transmissibles</jtitle><addtitle>Euro Surveill</addtitle><date>2020-06-25</date><risdate>2020</risdate><volume>25</volume><issue>25</issue><spage>10</spage><pages>10-</pages><issn>1560-7917</issn><issn>1025-496X</issn><eissn>1560-7917</eissn><abstract>The advent of COVID-19, has posed a risk that human respiratory samples containing human influenza viruses may also contain SARS-CoV-2. This potential risk may lead to SARS-CoV-2 contaminating conventional influenza vaccine production platforms as respiratory samples are used to directly inoculate embryonated hen's eggs and continuous cell lines that are used to isolate and produce influenza vaccines. We investigated the ability of these substrates to propagate SARS-CoV-2 and found that neither could support SARS-CoV-2 replication.</abstract><cop>Sweden</cop><pub>Centre Europeen pour la Surveillance Epidemiologique du SIDA (European Centre for the Epidemiological Monitoring of AIDS)</pub><pmid>32613937</pmid><doi>10.2807/1560-7917.ES.2020.25.25.2001122</doi><orcidid>https://orcid.org/0000-0002-7351-418X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1560-7917 |
| ispartof | Euro surveillance : bulletin européen sur les maladies transmissibles, 2020-06, Vol.25 (25), p.10 |
| issn | 1560-7917 1025-496X 1560-7917 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7331139 |
| source | PubMed Central |
| subjects | Animals Betacoronavirus Cell Line Chickens - immunology Chickens - virology Coronavirus - physiology Coronavirus Infections - epidemiology Coronavirus Infections - virology COVID-19 Dogs Eggs Humans Immunization Influenza Vaccines - immunology Influenza, Human - prevention & control Madin Darby Canine Kidney Cells Pandemics Pneumonia, Viral Rapid Communication Receptors, Virus - metabolism SARS-CoV-2 Severe Acute Respiratory Syndrome Severe acute respiratory syndrome coronavirus 2 Studies Vaccines Virology Virus Cultivation - methods Virus Replication Zoonoses |
| title | SARS-CoV-2 does not replicate in embryonated hen's eggs or in MDCK cell lines |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T07%3A52%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=SARS-CoV-2%20does%20not%20replicate%20in%20embryonated%20hen's%20eggs%20or%20in%20MDCK%20cell%20lines&rft.jtitle=Euro%20surveillance%20:%20bulletin%20europ%C3%A9en%20sur%20les%20maladies%20transmissibles&rft.au=Barr,%20Ian%20G&rft.date=2020-06-25&rft.volume=25&rft.issue=25&rft.spage=10&rft.pages=10-&rft.issn=1560-7917&rft.eissn=1560-7917&rft_id=info:doi/10.2807/1560-7917.ES.2020.25.25.2001122&rft_dat=%3Cproquest_pubme%3E2418147768%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c469t-c217c1cc44283ad08c4289050cec2dff7c2332a158e0c201a4f37bf63534008d3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2418147768&rft_id=info:pmid/32613937&rfr_iscdi=true |