Identification and functional analysis of five genes that encode distinct isoforms of protein phosphatase 1 in Nilaparvata lugens

• Published in: Scientific reports 2020-07, Vol.10 (1), p.10885, Article 10885
• Main Authors: Wang, Weixia, Zhu, Tingheng, Lai, Fengxiang, Wan, Pinjun, Wei, Qi, Fu, Qiang
• Format: Article
• Language: English
• Subjects: 5' Untranslated Regions - genetics; 631/208; 631/337; 631/61; Amino Acid Sequence; Animals; Base Sequence; Conserved Sequence; Developmental stages; DNA, Complementary - genetics; Double-stranded RNA; Eclosion; Enzyme Induction; Female; Fertility - drug effects; Fertilization; Gene expression; Genes, Insect; Hatchability; Hemiptera - enzymology; Hemiptera - genetics; Hemiptera - growth & development; Humanities and Social Sciences; Injection; Isoforms; Male; Mortality; multidisciplinary; Nilaparvata lugens; Oocytes - ultrastructure; Organ Specificity; Oviposition; Phosphoprotein phosphatase; Phosphorylation; Phylogeny; Protein Isoforms - genetics; Protein Isoforms - toxicity; Protein phosphatase; Protein Phosphatase 1 - genetics; Protein Phosphatase 1 - toxicity; Protein Processing, Post-Translational; Protein Subunits; Reproductive organs; RNA - genetics; RNA Interference; RNA-mediated interference; Science; Science (multidisciplinary); Sequence Alignment; Sequence Homology; Spermatogenesis; Vas Deferens - abnormalities
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-67735-7

Ten distinct cDNAs encoding five different protein phosphatases 1 (PPP1) were cloned from Nilaparvata lugens . NlPPP1α and NlPPP1β are highly conserved whereas NlPPP1-Y , NlPPP1-Y1 and NlPPP1-Y2 are lowly conserved among insects. NlPPP1α and NlPPP1β exhibited a ubiquitous expression, while NlPPP1-Y , NlPPP1-Y1 , and NlPPP1-Y2 were obviously detected from the 4th instar nymph to imago developmental stages in males, especially detected in internal reproductive organ and fat bodies of the male. Injection nymphs with dsRNA of NlPPP1α or NlPPP1β was able to reduce the target gene expression in a range of 71.5–91.0%, inducing a maximum mortality rate of 95.2% or 97.2% at 10th day after injection and eclosion ratio down by 65.5–100.0%. Injection with dsNlPPP1Ys targeted to NlPPP1-Y , NlPPP1-Y1 and NlPPP1-Y2 was able to induce a maximum mortality rate of 95.5% at 10th day after injection, eclosion ratio down by 86.4%. Knock-down one of the male-biased NlPPP1 genes has no effect on survival and eclosion ratio. Injection of 4th instar nymph with dsNlPPP1Ys led to reduced oviposition amount and hatchability, down by 44.7% and 19.6% respectively. Knock-down of NlPPP1-Y1 or NlPPP1-Y2 gene did not significantly affect oviposition amount but significantly affected hatchability. The results indicate that the male-biased NlPPP1 genes have overlapping functions in N. lugens development, and NlPPP1-Y1 and NlPPP1-Y2 may play important roles in spermatogenesis and fertilization. The dsNlPPP1β and dsNlPPP1Ys in this study could be the preferred sequence in RNAi and low-conserved male-biased NlPPP1 genes could be potential target for N. lugens control.