Molecular fingerprint of female bovine embryos produced in vitro with high competence to establish and maintain pregnancy

The objective was to identify the transcriptomic profile of in vitro-derived embryos with high competence to establish and maintain gestation. Embryos produced with X-sorted sperm were cultured from day 5 to day 7 in serum-free medium containing 10 ng/ml recombinant bovine colony-stimulating factor...

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Bibliographic Details
Published in:Biology of reproduction 2020-02, Vol.102 (2), p.292-305
Main Authors: Zolini, A.M, Block, J, Rabaglino, M.B, Tríbulo, P, Hoelker, M, Rincon, G, Bromfield, J.J, Hansen, P.J
Format: Article
Language:English
Subjects:
Animal reproduction
Biology
blastocyst
Cattle
CSF2
Embryo
embryonic survival
Embryos
Gene expression
Genetic aspects
Health aspects
Physiological aspects
Pregnancy
Reproductive health
RESEARCH ARTICLE
transcriptome
Ultrasonic imaging
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Summary:The objective was to identify the transcriptomic profile of in vitro-derived embryos with high competence to establish and maintain gestation. Embryos produced with X-sorted sperm were cultured from day 5 to day 7 in serum-free medium containing 10 ng/ml recombinant bovine colony-stimulating factor 2 (CSF2) or vehicle. The CSF2 was administered because this molecule can increase blastocyst competence for survival after embryo transfer. Blastocysts were harvested on day 7 of culture and manually bisected. One demi-embryo from a single blastocyst was transferred into a synchronized recipient and the other half was used for RNA-seq analysis. Using P < 0.01 and a fold change >2-fold or
ISSN:0006-3363
1529-7268
DOI:10.1093/biolre/ioz190