Discriminating stress from rest based on resting‐state connectivity of the human brain: A supervised machine learning study

• Published in: Human brain mapping 2020-08, Vol.41 (11), p.3089-3099
• Main Authors: Zhang, Wei, Llera, Alberto, Hashemi, Mahur M., Kaldewaij, Reinoud, Koch, Saskia B. J., Beckmann, Christian F., Klumpers, Floris, Roelofs, Karin
• Format: Article
• Language: English
• Subjects: acute stress; Amygdala; Brain; Brain mapping; Classification; Cortex (cingulate); Cortex (parietal); Corticosteroids; Cortisol; default mode network; Executive function; Filters; functional connectivity; Functional magnetic resonance imaging; Learning algorithms; Linear filters; Machine learning; Magnetic resonance imaging; Neural networks; Neuroimaging; Psychopathology; resting‐state functional magnetic resonance imaging; salience network; stress effects; Stress response
• ISSN: 1065-9471; 1097-0193
• DOI: 10.1002/hbm.25000

Acute stress induces large‐scale neural reorganization with relevance to stress‐related psychopathology. Here, we applied a novel supervised machine learning method, combining the strengths of a priori theoretical insights with a data‐driven approach, to identify which connectivity changes are most prominently associated with a state of acute stress and individual differences therein. Resting‐state functional magnetic resonance imaging scans were taken from 334 healthy participants (79 females) before and after a formal stress induction. For each individual scan, mean time‐series were extracted from 46 functional parcels of three major brain networks previously shown to be potentially sensitive to stress effects (default mode network (DMN), salience network (SN), and executive control networks). A data‐driven approach was then used to obtain discriminative spatial linear filters that classified the pre‐ and post‐stress scans. To assess potential relevance for understanding individual differences, probability of classification using the most discriminative filters was linked to individual cortisol stress responses. Our model correctly classified pre‐ versus post‐stress states with highly significant accuracy (above 75%; leave‐one‐out validation relative to chance performance). Discrimination between pre‐ and post‐stress states was mainly based on connectivity changes in regions from the SN and DMN, including the dorsal anterior cingulate cortex, amygdala, posterior cingulate cortex, and precuneus. Interestingly, the probability of classification using these connectivity changes were associated with individual cortisol increases. Our results confirm the involvement of DMN and SN using a data‐driven approach, and specifically single out key regions that might receive additional attention in future studies for their relevance also for individual differences.