Lethal Outcome of Leptospirosis in Southern Russia: Characterization of Leptospira Interrogans Isolated from a Deсeased Teenager

This article describes a lethal case of leptospirosis that occurred in Southern Russia. The Leptospira strain was isolated and characterized using a microscopic agglutination test, MALDI-TOF mass spectrometry, targeted PCR, and high-throughput sequencing. We show that molecular and mass-spectrometry...

Full description

Saved in:
Bibliographic Details
Published in:International journal of environmental research and public health 2020-06, Vol.17 (12), p.4238
Main Authors: Samoilov, Andrei E, Stoyanova, Nataliya A, Tokarevich, Nikolai K, Evengard, Birgitta, Zueva, Elena V, Panferova, Yulia A, Ostankova, Yulia V, Zueva, Elena B, Valutite, Diana E, Kovalev, Evgeniy V, Litovko, Anna R, Goncharov, Alexey U, Semenov, Alexandr V, Khafizov, Kamil, Dedkov, Vladimir G
Format: Article
Language:English
Subjects:
Abdomen
Adenoviruses
Agglutination
Analgesics
Case Report
Climate change
Deoxyribonucleic acid
DNA
Epidemics
Etiology
Fever
Genetic testing
Genomes
Hepatitis
Infections
Laboratories
Leptospira
Leptospirosis
Library collections
Mass spectrometry
Mass spectroscopy
Medical research
Mortality
Next-generation sequencing
Scientific imaging
Serology
Spectroscopy
Zoonoses
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This article describes a lethal case of leptospirosis that occurred in Southern Russia. The Leptospira strain was isolated and characterized using a microscopic agglutination test, MALDI-TOF mass spectrometry, targeted PCR, and high-throughput sequencing. We show that molecular and mass-spectrometry methods can be an alternative to conventional methods of leptospirosis diagnostics and Leptospira study, which require highly qualified staff and can be performed only at specialized laboratories. We also report the first whole genome of L. interrogans isolated in Russia.
ISSN:1661-7827
1660-4601
DOI:10.3390/ijerph17124238