Cytotoxicity and Mutagenicity of Narrowband UVB to Mammalian Cells

Phototherapy using narrowband ultraviolet-B (NB-UVB) has been shown to be more effective than conventional broadband UVB (BB-UVB) in treating a variety of skin diseases. To assess the difference in carcinogenic potential between NB-UVB and BB-UVB, we investigated the cytotoxicity via colony formatio...

Full description

Saved in:
Bibliographic Details
Published in:Genes 2020-06, Vol.11 (6), p.646
Main Authors: Buglewicz, Dylan J, Mussallem, Jacob T, Haskins, Alexis H, Su, Cathy, Maeda, Junko, Kato, Takamitsu A
Format: Article
Language:English
Subjects:
Animals
CHO Cells
Cricetinae
Cricetulus
DNA Damage - genetics
DNA Damage - radiation effects
Humans
Mutagenesis - genetics
Mutagenesis - radiation effects
Mutagens - toxicity
Mutation - radiation effects
Pyrimidine Dimers - genetics
Pyrimidine Dimers - radiation effects
Skin - metabolism
Skin - radiation effects
Ultraviolet Rays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Phototherapy using narrowband ultraviolet-B (NB-UVB) has been shown to be more effective than conventional broadband UVB (BB-UVB) in treating a variety of skin diseases. To assess the difference in carcinogenic potential between NB-UVB and BB-UVB, we investigated the cytotoxicity via colony formation assay, genotoxicity via sister chromatid exchange (SCE) assay, mutagenicity via hypoxanthine phosphoribosyltransferase (HPRT) mutation assay, as well as cyclobutane pyrimidine dimer (CPD) formation and reactive oxygen species (ROS) generation in Chinese hamster ovary (CHO) and their NER mutant cells. The radiation dose required to reduce survival to 10% (D value) demonstrated BB-UVB was 10 times more cytotoxic than NB-UVB, and revealed that NB-UVB also induces DNA damage repaired by nucleotide excision repair. We also found that BB-UVB more efficiently induced SCEs and HPRT mutations per absorbed energy dosage (J/m ) than NB-UVB. However, SCE and HPRT mutation frequencies were observed to rise in noncytotoxic dosages of NB-UVB exposure. BB-UVB and NB-UVB both produced a significant increase in CPD formation and ROS formation ( < 0.05); however, higher dosages were required for NB-UVB. These results suggest that NB-UVB is less cytotoxic and genotoxic than BB-UVB, but can still produce genotoxic effects even at noncytotoxic doses.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes11060646