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Powdery Mildew-Induced Hormonal and Photosynthetic Changes in Barley Near Isogenic Lines Carrying Various Resistant Genes
The present work focused on the characterization of some physiological mechanisms activated upon powdery mildew inoculation of the susceptible barley cultivar Ingrid and its near-isogenic lines (NILs) carrying various resistant genes ( , and ). After inoculation with f. sp. ( ), measurements of leaf...
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| Published in: | International journal of molecular sciences 2020-06, Vol.21 (12), p.4536 |
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| creator | Saja, Diana Janeczko, Anna Barna, Balázs Skoczowski, Andrzej Dziurka, Michał Kornaś, Andrzej Gullner, Gábor |
| description | The present work focused on the characterization of some physiological mechanisms activated upon powdery mildew inoculation of the susceptible barley cultivar Ingrid and its near-isogenic lines (NILs) carrying various resistant genes (
,
and
). After inoculation with
f. sp.
(
), measurements of leaf reflectance and chlorophyll
fluorescence were performed 3 and 7 day post-inoculation (dpi), while hormone assays were made 7 dpi.
-inoculated resistant genotypes were characterized by lowered leaf reflectance parameters that correlated with carotenoids (CRI) and water content (WBI) in comparison to inoculated Ingrid. The PSII activity (i.e., Fv/Fm, ETo/CSm and P.I.
) strongly decreased in susceptible Ingrid leaves when the disease symptoms became visible 7 dpi. In Mla plants with visible hypersensitive spots the PSII activity decreased to a lesser extent. Inoculation resulted in a very slight decrease of photosynthesis at later stage of infection in Mlg plants, whereas in resistant mlo plants the PSII activity did not change. Chlorophyll
fluorescence measurements allowed presymptomatic detection of infection in Ingrid and Mla. Changes in the homeostasis of 22 phytohormones (cytokinins, auxins, gibberellins and the stress hormones JA, SA and ABA) in powdery mildew inoculated barley are discussed in relation to resistance against this biotrophic pathogen. |
| doi_str_mv | 10.3390/ijms21124536 |
| format | article |
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,
and
). After inoculation with
f. sp.
(
), measurements of leaf reflectance and chlorophyll
fluorescence were performed 3 and 7 day post-inoculation (dpi), while hormone assays were made 7 dpi.
-inoculated resistant genotypes were characterized by lowered leaf reflectance parameters that correlated with carotenoids (CRI) and water content (WBI) in comparison to inoculated Ingrid. The PSII activity (i.e., Fv/Fm, ETo/CSm and P.I.
) strongly decreased in susceptible Ingrid leaves when the disease symptoms became visible 7 dpi. In Mla plants with visible hypersensitive spots the PSII activity decreased to a lesser extent. Inoculation resulted in a very slight decrease of photosynthesis at later stage of infection in Mlg plants, whereas in resistant mlo plants the PSII activity did not change. Chlorophyll
fluorescence measurements allowed presymptomatic detection of infection in Ingrid and Mla. Changes in the homeostasis of 22 phytohormones (cytokinins, auxins, gibberellins and the stress hormones JA, SA and ABA) in powdery mildew inoculated barley are discussed in relation to resistance against this biotrophic pathogen.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms21124536</identifier><identifier>PMID: 32630603</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Apoptosis ; Ascomycota - drug effects ; Ascomycota - pathogenicity ; Auxins ; Barley ; Carotenoids ; Cereals ; Chlorophyll ; Chlorophyll A - metabolism ; Cultivars ; Cytokinins ; Disease ; Disease Resistance - genetics ; Fluorescence ; Fungi ; Fungicides ; Gene Expression Regulation, Plant - genetics ; Genes ; Genotypes ; Gibberellins ; Homeostasis ; Hordeum - genetics ; Hordeum - metabolism ; Inoculation ; Leaves ; Moisture content ; Pathogens ; Photosynthesis ; Photosynthesis - physiology ; Photosystem II ; Physiology ; Plant Diseases - genetics ; Plant Leaves - metabolism ; Plant Proteins - metabolism ; Powdery mildew ; Reflectance ; Signs and symptoms ; Water content</subject><ispartof>International journal of molecular sciences, 2020-06, Vol.21 (12), p.4536</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-8961f208372c4b98c8a4bd3c0aa657ad1e44e74eedf915b0330a9a6c48faf3563</citedby><cites>FETCH-LOGICAL-c412t-8961f208372c4b98c8a4bd3c0aa657ad1e44e74eedf915b0330a9a6c48faf3563</cites><orcidid>0000-0002-8265-6243 ; 0000-0001-6584-3250</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2418884493/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2418884493?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25731,27901,27902,36989,36990,44566,53766,53768,74869</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32630603$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saja, Diana</creatorcontrib><creatorcontrib>Janeczko, Anna</creatorcontrib><creatorcontrib>Barna, Balázs</creatorcontrib><creatorcontrib>Skoczowski, Andrzej</creatorcontrib><creatorcontrib>Dziurka, Michał</creatorcontrib><creatorcontrib>Kornaś, Andrzej</creatorcontrib><creatorcontrib>Gullner, Gábor</creatorcontrib><title>Powdery Mildew-Induced Hormonal and Photosynthetic Changes in Barley Near Isogenic Lines Carrying Various Resistant Genes</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>The present work focused on the characterization of some physiological mechanisms activated upon powdery mildew inoculation of the susceptible barley cultivar Ingrid and its near-isogenic lines (NILs) carrying various resistant genes (
,
and
). After inoculation with
f. sp.
(
), measurements of leaf reflectance and chlorophyll
fluorescence were performed 3 and 7 day post-inoculation (dpi), while hormone assays were made 7 dpi.
-inoculated resistant genotypes were characterized by lowered leaf reflectance parameters that correlated with carotenoids (CRI) and water content (WBI) in comparison to inoculated Ingrid. The PSII activity (i.e., Fv/Fm, ETo/CSm and P.I.
) strongly decreased in susceptible Ingrid leaves when the disease symptoms became visible 7 dpi. In Mla plants with visible hypersensitive spots the PSII activity decreased to a lesser extent. Inoculation resulted in a very slight decrease of photosynthesis at later stage of infection in Mlg plants, whereas in resistant mlo plants the PSII activity did not change. Chlorophyll
fluorescence measurements allowed presymptomatic detection of infection in Ingrid and Mla. Changes in the homeostasis of 22 phytohormones (cytokinins, auxins, gibberellins and the stress hormones JA, SA and ABA) in powdery mildew inoculated barley are discussed in relation to resistance against this biotrophic pathogen.</description><subject>Apoptosis</subject><subject>Ascomycota - drug effects</subject><subject>Ascomycota - pathogenicity</subject><subject>Auxins</subject><subject>Barley</subject><subject>Carotenoids</subject><subject>Cereals</subject><subject>Chlorophyll</subject><subject>Chlorophyll A - metabolism</subject><subject>Cultivars</subject><subject>Cytokinins</subject><subject>Disease</subject><subject>Disease Resistance - genetics</subject><subject>Fluorescence</subject><subject>Fungi</subject><subject>Fungicides</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>Genes</subject><subject>Genotypes</subject><subject>Gibberellins</subject><subject>Homeostasis</subject><subject>Hordeum - genetics</subject><subject>Hordeum - metabolism</subject><subject>Inoculation</subject><subject>Leaves</subject><subject>Moisture content</subject><subject>Pathogens</subject><subject>Photosynthesis</subject><subject>Photosynthesis - physiology</subject><subject>Photosystem II</subject><subject>Physiology</subject><subject>Plant Diseases - genetics</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Proteins - metabolism</subject><subject>Powdery mildew</subject><subject>Reflectance</subject><subject>Signs and symptoms</subject><subject>Water content</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNpdkc1v1DAQxSMEoqVw44wsceFAwF9xnAsSrKBdaYEKAVdr1p7sepXYxU6o8t_jqqVaOM1I76enmfeq6jmjb4To6Ft_GDNnjMtGqAfVKZOc15Sq9uHRflI9yflAKRe86R5XJ4IrQRUVp9VyGa8dpoV89oPD63od3GzRkYuYxhhgIBAcudzHKeYlTHucvCWrPYQdZuID-QBpwIV8QUhkneMOQ9E3PhR1BSktPuzIT0g-zpl8w-zzBGEi51iAp9WjHoaMz-7mWfXj08fvq4t68_V8vXq_qa1kfKp1p1jPqRYtt3LbaatBbp2wFEA1LTiGUmIrEV3fsWZLhaDQgbJS99CLRomz6t2t79W8HdFZDFOCwVwlP0JaTARv_lWC35td_G1a0XCtZDF4dWeQ4q8Z82RGny0OAwQsfxkuS_xMtQ0v6Mv_0EOcU4nxhmJaayk7UajXt5RNMeeE_f0xjJqbTs1xpwV_cfzAPfy3RPEH6GmfBw</recordid><startdate>20200625</startdate><enddate>20200625</enddate><creator>Saja, Diana</creator><creator>Janeczko, Anna</creator><creator>Barna, Balázs</creator><creator>Skoczowski, Andrzej</creator><creator>Dziurka, Michał</creator><creator>Kornaś, Andrzej</creator><creator>Gullner, Gábor</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8265-6243</orcidid><orcidid>https://orcid.org/0000-0001-6584-3250</orcidid></search><sort><creationdate>20200625</creationdate><title>Powdery Mildew-Induced Hormonal and Photosynthetic Changes in Barley Near Isogenic Lines Carrying Various Resistant Genes</title><author>Saja, Diana ; 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,
and
). After inoculation with
f. sp.
(
), measurements of leaf reflectance and chlorophyll
fluorescence were performed 3 and 7 day post-inoculation (dpi), while hormone assays were made 7 dpi.
-inoculated resistant genotypes were characterized by lowered leaf reflectance parameters that correlated with carotenoids (CRI) and water content (WBI) in comparison to inoculated Ingrid. The PSII activity (i.e., Fv/Fm, ETo/CSm and P.I.
) strongly decreased in susceptible Ingrid leaves when the disease symptoms became visible 7 dpi. In Mla plants with visible hypersensitive spots the PSII activity decreased to a lesser extent. Inoculation resulted in a very slight decrease of photosynthesis at later stage of infection in Mlg plants, whereas in resistant mlo plants the PSII activity did not change. Chlorophyll
fluorescence measurements allowed presymptomatic detection of infection in Ingrid and Mla. Changes in the homeostasis of 22 phytohormones (cytokinins, auxins, gibberellins and the stress hormones JA, SA and ABA) in powdery mildew inoculated barley are discussed in relation to resistance against this biotrophic pathogen.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>32630603</pmid><doi>10.3390/ijms21124536</doi><orcidid>https://orcid.org/0000-0002-8265-6243</orcidid><orcidid>https://orcid.org/0000-0001-6584-3250</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of molecular sciences, 2020-06, Vol.21 (12), p.4536 |
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| language | eng |
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| source | Publicly Available Content Database; PubMed Central |
| subjects | Apoptosis Ascomycota - drug effects Ascomycota - pathogenicity Auxins Barley Carotenoids Cereals Chlorophyll Chlorophyll A - metabolism Cultivars Cytokinins Disease Disease Resistance - genetics Fluorescence Fungi Fungicides Gene Expression Regulation, Plant - genetics Genes Genotypes Gibberellins Homeostasis Hordeum - genetics Hordeum - metabolism Inoculation Leaves Moisture content Pathogens Photosynthesis Photosynthesis - physiology Photosystem II Physiology Plant Diseases - genetics Plant Leaves - metabolism Plant Proteins - metabolism Powdery mildew Reflectance Signs and symptoms Water content |
| title | Powdery Mildew-Induced Hormonal and Photosynthetic Changes in Barley Near Isogenic Lines Carrying Various Resistant Genes |
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