In vivo and in vitro observation of nasal ciliary motion in a guinea pig model

In vitro airway specimens are widely used to evaluate airway ciliary function. However, the function of in vitro ciliated cells may be far different from their actual in vivo physiological conditions. Due to the lack of a valid technique, direct images of in vivo airway ciliary motion have never bee...

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Bibliographic Details
Published in:Experimental biology and medicine (Maywood, N.J.) N.J.), 2020-06, Vol.245 (12), p.1039-1048
Main Authors: Pang, Chuan, An, Fengwei, Yang, Shiming, Yu, Ning, Chen, Daishi, Chen, Lei
Format: Article
Language:English
Subjects:
Animals
Cilia - physiology
Guinea Pigs
Models, Animal
Motion
Nasal Mucosa - physiology
Nose - physiology
Original Research
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Summary:In vitro airway specimens are widely used to evaluate airway ciliary function. However, the function of in vitro ciliated cells may be far different from their actual in vivo physiological conditions. Due to the lack of a valid technique, direct images of in vivo airway ciliary motion have never been captured and analyzed before. This study aims to examine nasal ciliary motion in living guinea pigs with comparison to in vitro observation. Nasal septum mucosa was exposed in anaesthetized guinea pigs and directly examined using a digital microscopy system. The study included three parts: (1) measurement of ciliary beat frequency (CBF) of nasal mucosa at room temperature in living guinea pigs and immediately after death, and in dissected mucosa specimens/cells for comparison; (2) monitoring of nasal ciliary motion, CBF, and ciliary beat distance (CBD) over 12 h in both living guinea pigs and dissected mucosa specimens/cells; and (3) measurement of ciliary motion changes in responses to temperature variations. Compared with when the animal was alive, the CBF after death and in dissected mucosa specimens/cells was lower by about 20% (P 
ISSN:1535-3702
1535-3699
DOI:10.1177/1535370220926443