Changes in the Microbiome of Mariculture Feed Organisms after Treatment with a Potentially Probiotic Strain of Phaeobacter inhibens

• Published in: Applied and environmental microbiology 2020-07, Vol.86 (14)
• Main Authors: Dittmann, Karen K, Rasmussen, Bastian Barker, Melchiorsen, Jette, Sonnenschein, Eva C, Gram, Lone, Bentzon-Tilia, Mikkel
• Format: Article
• Language: English
• Subjects: Algae; Alliances; Animal Feed; Animals; Antibacterial materials; Aquaculture; Bacteria - isolation & purification; Bacterial diseases; Biotechnology; Chlorophyta - microbiology; Community structure; Copepoda - growth & development; Copepoda - microbiology; Eggs; Fish; Fish eggs; Flatfishes - growth & development; Flatfishes - microbiology; Gene sequencing; Larva - microbiology; Larvae; Marine aquaculture; Microalgae - microbiology; Microbial activity; Microbiomes; Microbiota; Microorganisms; Organisms; Ovum - microbiology; Pathogens; Probiotics; Probiotics - pharmacology; Relative abundance; Rhodobacteraceae - chemistry; RNA, Bacterial - analysis; RNA, Ribosomal, 16S - analysis; rRNA 16S; Scophthalmus maximus; Taxa
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.00499-20

The genus has been explored as probiotics in mariculture as a sustainable strategy for the prevention of bacterial infections. Its antagonistic effect against common fish pathogens is predominantly due to the production of the antibacterial compound tropodithietic acid (TDA), and TDA-producing strains have repeatedly been isolated from mariculture environments. Despite many trials targeting pathogens, little is known about its impact on host-associated microbiomes in mariculture. Hence, the purpose of this study was to investigate how the addition of a TDA-producing strain affects the microbiomes of live feed organisms and fish larvae. We used 16S rRNA gene sequencing to characterize the bacterial diversity associated with live feed microalgae ( ), live feed copepod nauplii ( ), and turbot ( ) eggs/larvae. The microbial communities were unique to the three organisms investigated, and the addition of the probiotic bacterium had various effects on the diversity and richness of the microbiomes. The structure of the live feed microbiomes was significantly changed, while no effect was seen on the community structure associated with turbot larvae. The changes were seen primarily in particular taxa. The order was indigenous to all three microbiomes and decreased in relative abundance when was introduced in the copepod and turbot microbiomes, while it was unaffected in the microalgal microbiome. Altogether, the study demonstrates that the addition of in higher concentrations, as part of a probiotic regime, does not appear to cause major imbalances in the microbiome, but the effects were specific to closely related taxa. This work is an essential part of the risk assessment of the application of roseobacters as probiotics in mariculture. It provides insights into the impact of TDA-producing on the commensal bacteria related to mariculture live feed and fish larvae. Also, the study provides a sequencing-based characterization of the microbiomes related to mariculture-relevant microalga, copepods, and turbot larvae.