A 3D engineered scaffold for hematopoietic progenitor/stem cell co-culture in vitro

Proliferation of HPSCs in vitro can promote its broad clinical therapeutic use. For in vitro co-culture, interaction between the stem cell and feeder cell as well as their spatial position are essential. To imitate the natural microenvironment, a 3D engineered scaffold for CD34 + cells co-culture wa...

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Bibliographic Details
Published in:Scientific reports 2020-07, Vol.10 (1), p.11485-11485, Article 11485
Main Authors: Zhou, Dezhi, Chen, Lidan, Ding, Jinju, Zhang, Xiuxiu, Nie, Zhenguo, Li, Xinda, Yang, Bin, Xu, Tao
Format: Article
Language:English
Subjects:
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Antigens, CD34 - genetics
Bioprinting
CD18 antigen
CD34 antigen
CD38 antigen
Cell culture
Cell Proliferation - genetics
Coculture Techniques - methods
Fetal Blood - cytology
Flow Cytometry
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells - cytology
Hemopoiesis
Humanities and Social Sciences
Humans
Hydrogels
Hydrogels - chemistry
Hydrogels - pharmacology
Mesenchymal Stem Cells - cytology
multidisciplinary
Printing, Three-Dimensional
Receptors, Cell Surface - genetics
Ribonucleic acid
RNA
Science
Science (multidisciplinary)
Stem cells
Tissue Scaffolds - chemistry
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Summary:Proliferation of HPSCs in vitro can promote its broad clinical therapeutic use. For in vitro co-culture, interaction between the stem cell and feeder cell as well as their spatial position are essential. To imitate the natural microenvironment, a 3D engineered scaffold for CD34 + cells co-culture was established via 3D bioprinting. Herein, the concentration of hydrogel and the ratio of two kinds of cells were optimized. Flow cytometry, real time PCR and RNA-seq technology were applied to analyze the effect of the engineered scaffold on expanded cells. After 10 days co-culture with the engineered scaffold, the expansion of CD34 + CD38 − cells can reach 33.57-folds and the expansion of CD34 + CD184 + cells can reach 16.66-folds. Result of PCR and RNA-seq indicates that the CD34 + cells in 3D group exhibited a tendency of interaction with the engineered scaffold. Compared to 2D co-culture, this customizable 3D engineered scaffold can provide an original and integrated environment for HPSCs growth. Additionally, this scaffold can be modified for different cell co-culture or cell behavior study.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-68250-5