The Combination of IgA and IgG autoantibodies against Transcriptional Intermediary Factor-1γ contributes to the early diagnosis of Lung Cancer

• Published in: International journal of medical sciences 2020-01, Vol.17 (11), p.1561-1568
• Main Authors: Yu, Lili, Lin, Xiaoqing, Zhang, Liangming, Wu, Qingwei, Zhang, Songgao, Chen, Dunyan, Pan, Xiaojie, Huang, Yi
• Format: Article
• Language: English
• Subjects: Antibodies; Antibodies, Anti-Idiotypic - immunology; Antigens; Autoantibodies - immunology; Biomarkers, Tumor - immunology; Blotting, Western; Breast cancer; Chronic obstructive pulmonary disease; Colorectal cancer; Early Detection of Cancer; Enzyme-Linked Immunosorbent Assay; Enzymes; Female; Humans; Immunoglobulin A - immunology; Immunoglobulin E - immunology; Immunoglobulin G - immunology; Immunohistochemistry; Laboratories; Lung cancer; Lung Neoplasms - diagnosis; Lung Neoplasms - immunology; Male; Medical prognosis; Middle Aged; Proteins; Research Paper; Surgery; Tuberculosis
• ISSN: 1449-1907; 1449-1907
• DOI: 10.7150/ijms.47463

The aberrant expression of tumor-associated antigens (TAAs) is responsible for the release of large amounts of autoantibodies in sera, and serum autoantibody detection has been demonstrated to contribute to the early diagnosis of malignancies. Recent studies showed the closely correlation of transcriptional intermediary factor-1γ (TIF1γ) with some malignancies. In our pilot study, we found aberrantly high expression of TIF1γ protein existed in cancer tissues other than matched paracancerous tissues of patients with lung cancer (LC) at early stage by immunohistochemistry (IHC) staining. As a result, this study aims to detect the expression of autoantibodies against TIF1γ in sera of patients with LC at early stage by using enzyme-linked immunosorbent assay (ELISA) and investigate its potential value for the early diagnosis of LC. The expressions of TIF1γ protein in 60 pairs of LC tissues and matched paracancerous tissues were detected by IHC staining. The levels of anti-TIF1γ-IgA, IgG, IgM, and IgE in the sera of 248 patients with LC at early stage, 200 patients with lung benign lesions (LBL), and 218 healthy controls (HC) were detected by ELISA, respectively. Western blot was used to validate the ELISA results of serum autoantibodies against TIF1γ. The positive rate of TIF1γ protein expression in LC tissues was 83.33%, which was significantly higher than 25.00% in paracancerous tissues ( 0.05), while the levels and positive rates of anti-TIF1γ-IgA and anti-TIF1γ-IgG were significantly higher than that in LBL group and HC group (