Ginsenoside Rh1 Alleviates HK-2 Apoptosis by Inhibiting ROS and the JNK/p53 Pathways

Background. Cisplatin is widely used in the treatment of malignant patients; however, its adverse nephrotoxic effects limit its clinical use. Ginsenoside Rh1 is a main component of ginseng and has many pharmaceutical effects, including immunomodulatory effects. Objective. The objective of this resea...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2020, Vol.2020 (2020), p.1-8
Main Authors: Yang, Qi, Zhang, Song, Qian, Lin
Format: Article
Language:English
Subjects:
Apoptosis
Care and treatment
Caspase-3
Chinese medicine
Cholecystokinin
Cisplatin
Drug dosages
Experiments
Ginseng
Ginsenosides
Immunomodulation
Kidneys
p53 Protein
Patients
Propidium iodide
Proteins
Signal transduction
Stains & staining
Tumor proteins
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Summary:Background. Cisplatin is widely used in the treatment of malignant patients; however, its adverse nephrotoxic effects limit its clinical use. Ginsenoside Rh1 is a main component of ginseng and has many pharmaceutical effects, including immunomodulatory effects. Objective. The objective of this research is to assess the effects of ginsenoside Rh1 on a cisplatin-induced HK-2 injury model and to study its potential effect mechanisms. Methods. HK-2 cell vitality was assessed via Cell Counting Kit-8 (CCK-8) assay. Carboxyfluorescein succinimidyl ester/propidium iodide (CFSF/PI) staining was used to detect the apoptosis of HK-2 cells. ROS expression was detected by DCFDA. The expressions of JNK, p53, caspase-3, Bax, and NGAL were detected by western blot. Results. Ginsenoside Rh1 was found to increase the vitality of HK-2 cells and inhibit ROS production and the apoptosis of HK-2 cells in a cisplatin-induced injury model. Ginsenoside Rh1 was found to inhibit the expression of JNK, p53, caspase-3, Bax, and NGAL in a cisplatin-induced injury model. Conclusion. Ginsenoside Rh1 alleviated HK-2 apoptosis in a cisplatin-induced injury model by inhibiting ROS production and the JNK/p53 pathway. Ginsenoside Rh1 may be a promising drug for the alleviation of cisplatin-induced nephrotoxicity in malignant patients.
ISSN:1741-427X
1741-4288
DOI:10.1155/2020/3401067